牡丹根腐病病原的形态与分子鉴定  

Molecular and Morphological Identification of Peony Root Rot Pathogen

作　　者：成玉梅[1] 赵丹[2] 康业斌[3] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471003 [2]信阳师范学院华锐学院,河南信阳464000 [3]河南科技大学林学院,河南洛阳471003

出　　处：《北方园艺》2015年第16期116-119,共4页Northern Horticulture

基　　金：河南省科技厅重点科技攻关资助项目(102102110090)

摘　　要：以河南洛阳各大主要牡丹园栽培的牡丹典型根腐病病株为试材,采用组织分离法获得纯菌株,并对所得菌株进行致病性测定、形态学鉴定及rDNA-ITS序列分析,研究牡丹根腐病的病原菌种类。结果表明：分离获得的4个菌株在PDA培养基上均产生茂密、呈疏松棉絮状的菌丝及黄、红、紫等色素,大型分生孢子镰刀形或椭圆形,无色,多胞;小型分生孢子卵圆形至椭圆形,无色,单胞或双胞。致病性测定表明,4个菌株能侵染离体牡丹根尖使其变黑。在GenBank序列数据库中,F1～F4菌株的DNA序列与编号为HM214456.1和AB498917.1等的F.solani ITS区段DNA序列的同源性为99%～100%。据此,将河南洛阳牡丹根腐病的病原菌鉴定为F.solani。Taking the typical infected plants of peony root rot as test materials,which from in mainly peony garden in Luoyang,Henan Province.The pathogens were isolated by tissue separation,tested according to the standard Koch＇s postulation methods,and identified by the methods of microscopy and molecules,the pathogen species of peony root were studied.The results showed that four strains all produced thick,loose cotton wool hypha and yellow,red,violet colors in PDA.Macroconidium were falciform or ellipse,colourless,multi-cell;microconidia were oval to ellipse,colourless,one-celled or bicellular.those strains of Fusariumcould infect invitropeony root and make it black.The DNA sequence homology of F1 to F4strains and Nos in GenBank sequence database of HM214456.1and AB498917.1 F.solani was99%-100%.Accordingly,the pathogen of peony root rot disease was identified as F.solani.

关 键 词：牡丹 镰刀菌 形态鉴定 分子鉴定 

分 类 号：S436.81[农业科学—农业昆虫与害虫防治]