隐丹参酮对人胃癌细胞SGC-7901凋亡的影响及其机制  

Effect and mechanism of cryptotanshinone on apoptosis of human gastric cancer cell line SGC-7901

作　　者：邓凤春[1] 于占江[2] 杨钰[2] 赵红晔[3] 王滨[3] 周丽[3] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江161006 [2]齐齐哈尔医学院第三附属医院普外科 [3]齐齐哈尔医学院生理教研室

出　　处：《北京中医药大学学报》2015年第7期457-461,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(No.12531830)

摘　　要：目的探讨隐丹参酮(CPT)对人胃癌细胞SGC-7901凋亡的影响及其机制。方法用不同浓度的CPT作用于人胃癌细胞SGC-7901,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖的活力;流式细胞术检测细胞凋亡的变化;Real Time RT-PCR法检测细胞凋亡相关蛋白Bax、Bcl-2、p53和p21 mRNA表达的变化;Western blot法检测细胞凋亡相关蛋白Bax、Bcl-2、p53和p21表达的变化。结果 CPT作用于人胃癌细胞SGC-7901 24 h后,可明显抑制细胞生长,诱导细胞发生凋亡;在转录水平上调促细胞凋亡蛋白Bax和p53 mRNA的表达,下调抑制细胞凋亡蛋白Bcl-2 mRNA的表达;在翻译水平上调促细胞凋亡蛋白Bax和p53的表达,下调抑制细胞凋亡蛋白Bcl-2的表达。结论 CPT对人胃癌细胞SGC-7901具有诱导凋亡的作用,其机制可能与线粒体途径相关。Objective To investigate the apoptosis effect of cryptotanshinone （CPT） on human gastric cancer cell line SGC-7901, so as to explore the molecular mechanism related. Methods SGC-7901 ceils were treated with varying concentration of CPT. MTT assays and flow cytometry were used to evalu- ate the effects of CPT on the proliferation and apoptosis of SGC-7901 cells. The protein expressions of Bax, Bcl-2, p53 and p21, a series of apoptosis-related proteins, were observed by Western blotting, and the mRNA expressions of Bax, Bcl-2, p53 and p21 were determined by real-time RT-PCR. Results Fol- lowing 24 h treatment, CPT was capable of inhibiting proliferation and inducing apoptosis of SGC-7901 cells significantly. In addition, Bax and p53 mRNA and protein were upregulated, while Bcl-2 mRNA and protein were downregulated. It is well known that both Bcl2 and Bax are transcriptional targets for the tumour suppressor protein, p53, which induces cell cycle arrest or apoptosis. Conclusion Apoptosis can be induced in SGC-7901 cells by CPT, potentially via a mitochondrial pathway.

关 键 词：隐丹参酮 人胃癌细胞SGC-7901 细胞增殖 细胞凋亡 线粒体途径 

分 类 号：R285.5[医药卫生—中药学]