高效液相色谱法特征图谱用于玄参饮片的质量评价  

Quality Evaluation of Scrophulariae Slices Based on HPLC Specific Chromatogram

作　　者：朱月月 付静[1] 聂诗明[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《湖北中医药大学学报》2015年第4期29-34,共6页Journal of Hubei University of Chinese Medicine

基　　金：国家中药管理局项目(201007012-2-3)

摘　　要：目的建立玄参饮片的高效液相色谱法(HPLC)特征图谱,为玄参饮片的质量评价提供依据。方法采用高效液相色谱法,以C18(250mm×4.6mm,5μm)为色谱柱,甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱,以道地优质玄参为建模样本,建立玄参饮片的HPLC标准特征图谱。结果该特征图谱具18个共有特征峰。其中安格洛苷-C、肉桂酸和哈巴俄苷的色谱峰得到很好的分离,新产药材加工的13批饮片相似度均>0.95,其质量较好;23批市售饮片相似度有较大差别,存在较大质量差异。结论建立的玄参饮片HPLC标准特征图谱特征峰数量多,色谱信息量大,有助于玄参饮片的质量评价。Objective To establish the HPLC specific chromatogram of Scrophulariae slices for quality evaluation. Methods The HPLC specific chromatogram of Scrophulariae slices was developed. Diamansil C18（250mm × 4.6mm ×5μm） was adopted ; The mobile phase was the mixture of methanol and 0.1% formic acid water with a gradient mode; The HPLC specific chromatogram of Scrophulariae slices was determined by using high - quality geoherbs as the modeling sample. Results 18 co - possessing peaks were selected; The peaks of angelo glycoside - C, cinnamic acid and harpagoside were detached clearly; The similarities of 13 batches of new produced Scrophulariae slices were higher than O. 95 ; The similarities of 23 batches of Scrophulariae slices on sale differed greatly. Conclusion The HPLC specific chromatogram of Scrophulariae slices includes quantities of characteristic peaks and information, which contribute to the quality evaluation of Scrophulariae slices.

关 键 词：玄参饮片 HPLC 特征图谱 质量评价 

分 类 号：R284.2[医药卫生—中药学]