大菱鲆源鳗弧菌耐药表型及耐药基因检测  

Detection of Drug Resistance Phenotypes and Genes in Vibrio anguillarumfrom Turbot Scophthalmus maximus

作　　者：靳晓敏[1] 葛慕湘[1] 张艳英[1] 房海[1] 陈翠珍[1] 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《水产科学》2015年第8期510-514,共5页Fisheries Science

基　　金：河北省重点基础研究项目(11960503D);现代农业产业技术体系河北省创新团队建设项目;秦皇岛市科学技术研究与发展计划项目(201401A058)

摘　　要：对分离自大菱鲆、具有不同理化性质的30株致病性鳗弧菌，采用K—B纸片扩散法检测其耐药表型，PCR法检测p内酰胺类TEM基因、氨基糖苷类ant（3”）I基因、磺胺类Sull基因和四环素类tet（A）基因，分析其耐药基因与耐药表型间的关系，以查明大菱鲆源致病性鳗弧菌不同类型常见耐药基因的携带状况，并探讨耐药基因与耐药表型的相关性。结果表明：所检30株鳗弧菌均携带TEM、ant（3”）-I和Sull基因，但未检出tet（A）基因；所有菌株均具有多重耐药性，大部分菌株耐抗菌类药物达6～8种；耐药基因的携带与耐药表型间存在一定相关性，但并非完全对应。The drug-resistance was tested by Kirby-Bauer method, and four drug-resistance genes including TEM against β-lactamase genotypes, ant（3＂）- I against aminoglycoside, Sull against sulfonamides and tet （A） against tetracyclines were detected by PCR method in 30 bacterial strains isolated from turbot Scoph- thalmus rnaxirnus infected by Vibrio anguillarum to investigate the correlation between the drug resistance phenotypes and the drug resistance genotypes in V. anguillarum. The results showed that genes TEM, ant（3＂）- I and Sutl were detected in all strains tested except tet（A） gene in the experiment. All strains were found to be multi-drug resistant, most of which were resistant to 6--8 antibiotics. There was a cer tain relationship between the drug resistance phenotype and the related resistance genes, without entirely corresponding.

关 键 词：鳗弧菌 耐药性 耐药基因 

分 类 号：S917.1[农业科学—水产科学]