泰国葛不同部位几种次生代谢产物的研究  

Study of Secondary Metabolites from Different Parts of Pueraria mirifica

作　　者：马树运 楚纪明 李海峰[2] 徐立[1] 

机构地区：[1]大理学院基础医学院,云南大理671000 [2]大理学院药物研究所,云南大理671000

出　　处：《大理学院学报（综合版）》2015年第8期8-10,共3页Journal of Dali University

基　　金：云南省教育厅科研基金资助重点项目(2010Z1006)

摘　　要：目的:分析泰国葛根、茎、叶中葛根素、大豆苷和染料木素的含量,探讨植物最佳有效部位。方法:采用高效液相色谱法进行分析,色谱柱为Hypersil BDS C18柱(250 nm×4.6 mm,5μm);流动相为甲醇和水,梯度洗脱,流速0.7 m L/min,进样量10μL,柱温为25℃,检测波长250 nm。结果:泰国葛不同部位葛根素含量为:根(0.531±0.089)%>茎(0.024±0.006)%>叶(0.018±0.004)%;大豆苷含量为:根(0.226±0.034)%>叶(0.135±0.009)%>茎(0.041±0.006)%;染料木素含量为:根(0.075±0.001)%>茎(0.054±0.007)%>叶(0.016±0.003)%。结论:泰国葛以根的应用价值为最高,茎部的葛根素和染料木素含量优于叶,而叶大豆苷含量优于茎。Objective： To analyze the content of puerarin, daidzin and genistein from roots, stems and leaves of Pueraria mirifwa, and to explore the best effective parts. Methods： HPLC with the Hypersil BDS C18 column （250 mm×4.6 mm, 5 μm） was adopted. Mthanol and water were used as mobile phase of gradient elution, with the flow rate of 0.7 mL/min. The injection volume of the sample was 10 μL. The column temperature was 25 ℃, and the detection wavelength was 250 nm. Results： The descending order of puerarin content in the samples of different parts of P. mirifica was root （0.531 ± 0.089）% 〉 stem （0.024± 0.006）% 〉 leaf （0.018± 0.004）%. The descending order of daidzin content was root （0.226 ±0.034）% 〉 leaf （0.135 ± 0.009）% 〉 stem （0.041 ± 0.006）%. The descending order of genistein content was root （0.075 ±0.001 ）% 〉 stem （0.054 ±0.007）% 〉 leaf （0.016 ± 0.003）%. Conclusion： Values of the three secondary metabolites in roots of P. mirifica is the highest. Content of puerarin and genistein in stems are higher than them in leaves. The content of daidzin in leaves is higher than it in stems.

关 键 词：泰国葛 高效液相色谱法 不同部位 次生代谢产物 

分 类 号：R284.1[医药卫生—中药学]