脾气虚证大鼠脑肠肽动态变化及四君子汤干预效应研究  

Study On Dynamic Changes of Ghrelin of Spleen-qi-deficiency Rats and Intervention Effects of Sijunzi Decoction

作　　者：田茸[1] 巩子汉 杨晓轶[2] 朱立鸣[2] 段永强[2] 成映霞[2] 杜娟[2] 李兰珍[2] 王燕[2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]甘肃中医药大学,甘肃兰州730020

出　　处：《中国中医药信息杂志》2015年第9期68-71,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81160420);甘肃省自然科学研究基金计划(1010RJZA148);甘肃省教育厅项目(1006-05)

摘　　要：目的探讨四君子汤对脾气虚证的动态干预效应及作用机制。方法实验大鼠随机分为正常组、模型组、四君子汤组。除正常组外均以苦寒破气、游泳力竭双因素法复制脾气虚证模型,造模同时四君子汤组给予四君子汤20 g/(kg·d)干预,采用ELISA和放射免疫法检测不同时间点(14、21、28 d)模型大鼠小肠组织及血清中胃泌素(GAS)、胃动素(MTL)、生长抑素(SS)、血管活性肠肽(VIP)的活性,以及四君子汤的干预效应。结果与正常组比较,模型组大鼠血清和小肠组织中GAS和MTL降低、SS和VIP升高(P<0.05,P<0.01);与模型组比较,四君子汤组大鼠血清和小肠组织中GAS和MTL升高、SS和VIP降低(P<0.05,P<0.01)。结论脑肠肽在脾气虚证大鼠小肠组织和血清中呈现动态时相性一致的变化,四君子汤可能通过调节大鼠脑肠肽的活性从而发挥其治疗脾气虚证的作用。Objective To observe the dynamic changes of ghrelin of spleen-qi-deficiency rats and the intervention effects ofSijunzi Decoction.Methods Experimental rats were randomly divided into normal control group, model group andSijunzi Decoction group. Except for normal control group, spleen-qi-deficiency model was copied through the two-factor methods of breaking qi by bitter cold and swimming exhausted. Meanwhile,Sijunzi Decoction group was given 20 g/（kg？d）Sijunzi Decoction intervention. The activities of GAS, MTL, SS and VIP at different time points （14, 21, 28 d） in intestine and serum were detected by ELISA and RIA. At the same time the intervention effect of Sijunzi Decoction was studied.Results Compared with normal control group, GAS and MTL in intestine and serum of model rats decreased, while SS and VIP increased （P〈0.05,P〈0.01）. Compared with model group, GAS and MTL in intestine and serum of rats in theSijunzi Decoction group increased, while SS and VIP decreased （P〈0.05,P〈0.01）.Conclusion Ghrelin in intestine and serum of spleen-qi-deficiency rats shows dynamic coincidental changes.Sijunzi Decoction can treat spleen qi deficiency by regulating the activities of rat ghrelin.

关 键 词：脾气虚证 四君子汤 胃泌素 胃动素 生长抑素 血管活性肠肽 大鼠 

分 类 号：R285.5[医药卫生—中药学]