HPLC同时测定复方藤乌软膏中6种乌头类生物碱含量  

Simultaneous Determination of 6 Aconitum Alkaloids in Compound Tengwu Ointment by HPLC

作　　者：李美艳[1,2] 赫清雪[1,2] 王爱武[1] 

机构地区：[1]山东大学附属省立医院,山东济南250021 [2]山东中医药大学,山东济南250355

出　　处：《中国中医药信息杂志》2015年第9期87-90,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的：建立高效液相色谱法（HPLC）同时测定复方藤乌软膏中6种乌头类生物碱含量的方法。方法色谱柱为Agilent XDB-C18柱（250 mm×4.6 mm,5μm）；流动相A为乙腈-四氢呋喃（25∶8）,B为0.1 mol/L醋酸铵溶液（每1000 mL加冰醋酸0.5 mL）,梯度洗脱；流速：1 mL/min；检测波长：235 nm；柱温：25℃。结果乌头碱在0.072～0.648μg具有良好的线性关系（r＝0.9995）,平均回收率为99.29%,RSD＝1.25%；新乌头碱在0.062～0.648μg具有良好的线性关系（r＝0.9995）,平均回收率为99.12%,RSD＝0.85%；次乌头碱在0.064～0.384μg具有良好的线性关系（r＝0.9998）,平均回收率为99.57%,RSD＝1.07%；苯甲酰乌头原碱在0.056～0.672μg具有良好的线性关系（r＝0.9992）,平均回收率为98.11%,RSD＝0.61%；苯甲酰新乌头原碱在0.055～0.993μg具有良好的线性关系（r＝0.9999）,平均回收率为99.27%,RSD＝1.10%；苯甲酰次乌头原碱在0.078～0.702μg具有良好的线性关系（r＝0.9998）,平均回收率为99.08%,RSD＝1.38%。结论本方法操作简便、灵敏度高、重复性好,结果准确,可作为复方藤乌软膏中乌头类生物碱的含量测定方法。Objective To establish an HPLC method for the simultaneous determination of the 6 aconitum alkaloids in compoundTengwu Ointment.Methods Chromatographic column was Aglient XDB-C18 column （4.6 mm × 250 mm, 5μm）, with the mobile phase A of acetonitrile- tetrahydrofuran （25∶8）, phase B was 0.1 mol/L ammonium acetate solution （0.5 mL acetic acid added to the 1000 mL） with gradient elution;the flow rate was 1 mL/min;the detection wavelength was set at 235 nm;the column temperature was 25℃.Results Aconitine was in the good linear range of 0.072-0.648μg （r=0.999 5）, with the average recovery of 99.29%, RSD=1.25%. Mesaconitine was in the good linear range of 0.062-0.648μg （r=0.999 5）, with the average recovery of 99.12%, RSD=0.85%. Hypaconitine was in the good linear range of 0.064-0.384μg （r=0.999 8）, with the average recovery of 99.57%, RSD=1.07%. Benzoylaconine was in the good linear range of 0.056-0.672μg （r=0.999 2）, with the average recovery of 98.11%, RSD=0.61%. Benzoylmesaconine was in the good linear range of 0.055-0.993μg （r=0.999 9）, with the average recovery of 99.27%, RSD=1.10%. Benzoylhypaconine was in the good linear range of 0.078-0.702μg （r=0.999 8）, with the average recovery of 99.08%, RSD=1.38%.Conclusion This method is simple, sensitive, repeatable and accurate, which can be used for determination of aconitum alkaloids in compoundTengwu Ointment.

关 键 词：复方藤乌软膏 乌头碱 新乌头碱 次乌头碱 苯甲酰乌头原碱 苯甲酰新乌头原碱 苯甲酰次乌头原碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]