角蛋白金属结合体的制备及清除超氧阴离子自由基性能  

Preparation of keratin conjugating with metal complexes and its activity for scavenging superoxide anion free radical

作　　者：徐知丽 何玉凤[1] 冯瑞丽[1] 李晓晓[1] 闫桂芳[1] 王荣民[1] 

机构地区：[1]生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2015年第8期1174-1178,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21263024,21364012)资助

摘　　要：本文在制备可溶性羽毛角蛋白溶液的基础上,制备了羽毛角蛋白(FK)结合ZnII、CuII、MnII和NiII的角蛋白金属结合体(FKM,M=Zn,Cu,Mn,Ni)。采用IR、UV-Vis、TGA、CD和SEM等对其结构进行了表征和分析。用核黄素/蛋氨酸-NBT还原法考察了角蛋白金属结合体FKM清除超氧阴离子自由基(O2-)的性能,探讨了其清除机理。结果表明:含不同金属离子的结合体均具有清除O2-的能力。其中,Cu结合体的抗氧化活性最强。The water-soluble biopolymer, feather keratin-metal conjugates （ FKM ）, were prepared by the soluble feather keratin （FK） conjugating with metal ion （ Zn＂, Cu＂, Mn＂ , Ni＂ ） . The structures of FKM were characterized by IR, UV-Vis, TGA, CD and SEM. The superoxide anion radical （ O2^＊- ） scavenging activity of FKM was investigated by NBT photochemical reduction and the effect mechanism of scavenging O2^＊-was also explored. The results show that the conjugates with different metal ions had antioxidant activities. After combining with the biopolymer FK, the Cu conjugate （FKCu）shows the most excellent scavenging ability of superoxide anion radical （ O2^＊-）.

关 键 词：羽毛角蛋白 水溶性生物高分子 清除超氧阴离子自由基 SOD模拟物 

分 类 号：O629.33[理学—有机化学]