六氢β-酸月桂酸酯在鸡体内的药动学研究  

Study on pharmacokinetics of hexahydrolupulon laurale in chickens

作　　者：彭险峰 黄计军 叶晓岚 李俊健 任阳 姜美华 区煜荣 

机构地区：[1]广州英赛特生物技术有限公司,广东广州510663

出　　处：《中国饲料》2015年第16期20-21,24,共3页China Feed

基　　金：国家科技部中小企业技术创新项目(14C26214402670);广州市中小企业创新基金(151000098)

摘　　要：为研究六氢β-酸月桂酸酯在鸡体内的药动学,本试验选用12只健康鸡,随机分为2组,分别单剂量40mg/kg体重灌胃给药和1 mg/kg体重静脉注射给药。在给药前后不同时间点从前腔静脉采集血样,分离血浆,用高效液相色谱法测定血浆中六氢β-酸月桂酸酯的浓度。用Winnonlin 5.2.1的非房室模型处理血浆药物浓度-时间数据。单剂量口服六氢β-酸月桂酸酯的主要药动学参数:Cmax(0.04±0.01)μg/m L,tmax(1.00±0.15)h,T1/2(2.35±0.17)h,AUC(0.13±0.01)μg·h/m L,V(1024.1±98.4)L/kg,CLB(302.2±7.6)L/h·kg,MRT(3.55±0.27)h;单剂量静脉注射六氢β-酸月桂酸酯的主要药动学参数:C0(1.25±0.17)μg/m L,T1/2(1.10±0.25)h,AUC(0.82±0.14)μg·h/m L,V(1.93±0.13)L/kg,CLB(1.25±0.21)L/h·kg,MRT(1.27±0.31)h。结果表明:灌胃六氢β-酸月桂酸酯与静脉注射六氢β-酸月桂酸酯相比,鸡灌胃给药的生物利用度为0.4%,灌胃给药后吸收极少,静脉注射血药浓度较高,消除半衰期均较短。Pharmacokinetics of hexahydrolupulon laurate were investigated by oral administration at a single dosage of 40 mg/kg body weight and intravenous injection at a single dosage of 1 mg/kg body weight in chickens.A total of 12 chickens were divided randomly into two groups for the experiment,respectively.Blood samples were collected at different intervals after administration.The hexahydrolupulon laurate concentration in plasma of all groups was determined by high performance liquid chromatography （HPLC）.The plasma concentration-time data of hexahydrolnpulon laurate was ana- lyzed by Winnonlin 5.2. l.The main pharmacokinetic parameters of hexahydrolupulon laurate after oral administration were as follows ： Cmax （0.04±0.01） μg/mL, tmax （ 1.00±0.15 ）h, T1/2 （2.35 ±0.17 ）h ,AUC （0.13 ±0.01 ）μg. h/mL, V （ 1024.1 ±98.4）L/ kg, CL50 （ 302.2±7.6 ）L/h- kg, MRT （3.55 ±0.27 ）h.The main pharmacokinetic parameters of hexahydrolupulon laurate after intravenous injection administration were as follows ： C, （ 1.25±0.17 ） μg/mL, T1/2 （ 1.10±0.25 ）h, AUC （0.82±0.14） μg h/ mL,V （1.93±0.13）L/kg,CLB （1.25±0.21）L/h.kg,MRT （1.27±0.31）h.The results showed that the bioavailability was 0.4% after oral administration of hexahydrolupulon laurate （40 mg/kg body weight）in chickens.The absorption was very lit- tle, and the concentration of blood druy of intravenous injection administration was higher than oral administration, but the elimination half-life was all the same short.

关 键 词：六氢β-酸月桂酸酯 鸡 药动学 高效液相色谱法 

分 类 号：S816.7[农业科学—饲料科学]