PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤保护作用的实验研究  

Experimental research on the protective effect of PPARγagonist rosiglitazone on myocardial reperfusion injury

作　　者：袁博[1] 王东琦[2] 刘胜强 任晖[1] 巩洁[1] 王蕊[1] 高天林[1] 

机构地区：[1]陕西省安康市中心医院心内科一病区,陕西安康725000 [2]西安交通大学附属第一医院心内科,陕西西安710061 [3]安康市中医医院心内科,陕西安康725000

出　　处：《海南医学院学报》2015年第8期1014-1017,共4页Journal of Hainan Medical University

基　　金：2014年安康市科技研究发展计划项目(2014AK03-11)~~

摘　　要：目的:研究PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤的保护作用。方法:选择SD大鼠进行研究,随机分为假手术组、缺血再灌注损伤组、罗格列酮治疗组,检测心肌梗死面积、细胞凋亡指数、心肌功能指标以及凋亡相关分子的mRNA含量。结果:I/R组大鼠的梗死面积、凋亡指数大于Sham组,ROC组大鼠的梗死面、凋亡指数小于I/R组;I/R组大鼠血浆中CK-MB、cTnT含量高于Sham组,ROC组大鼠血浆中CK-MB、cTnT含量低于I/R组;I/R组大鼠心肌组织中β-MHC/α-MHC比例、bax和caspase-3含量高于Sham组,bcl-2和miR-208a的含量低于Sham组;ROC组大鼠心肌组织中β-MHC/α-MHC比例、bax和caspase-3含量低于I/R组,bcl-2和miR-208a的含量高于I/R组。结论:PPARγ激动剂罗格列酮对大鼠心肌再灌注损伤具有保护作用,能够缩小梗死面积、减少梗死细胞数目、调节促凋亡和抗凋亡基因的表达。Objective; To explore the protective effects of PPAR7 agonist rosiglitazone on myocardial reperfusion inju- ry. Methods： SD rats were chosen and randomly divided into sham group, ischemia-reperfusion injury group and rosiglitazone treatment group. Then myocardial infarct size, apoptotic index, myocardial performance index and mRNA contents of apopto- sis-related molecules were detected. Results Infarct size and apoptotic index of I/R group were greater than those of Sham group, but infarct size and apoptotic index of ROC group were less than those of I/R group. CK-MB and cTnT contents in plasma of I/R group were higher than those of Sham group, and CK-MB and cTnT contents in plasma of ROC group were low- er than those of I/R group. β-MHC/α-MHC proportion as well as bax and caspase-3 contents in myocardial tissue of I/R group were higher than those of Sham group, and bcl-2 and miR-208a contents were lower than those of Sham group β-MHC/a-MHC/α proportion as well as bax and caspase-3 contents in myocardial tissue of ROC group were lower than those of I/R group, and bcl-2 and miR 208a contents were higher than those of I/R group. Conclusion= PPARy agonist rosiglitazone shows protec- tive effect on myocardial reperfusion injury; it can reduce the infarct size, decrease the number of infarction cells and regulate the expression of pro-apoptotic and anti-apoplotic genes.

关 键 词：过氧化物酶体增殖物激活受体 缺血再灌注损伤 罗格列酮 凋亡 

分 类 号：R977.15[医药卫生—药品]