子宫内膜炎对NF-кB信号通路炎症蛋白和氧化因子的影响  

Effect of endometritis on level of inflammatory protein and oxidation factor via NF-κB signaling pathway

作　　者：倪茗[1] 

机构地区：[1]武汉科技大学附属汉阳医院妇产科,湖北武汉430050

出　　处：《海南医学院学报》2015年第8期1023-1026,共4页Journal of Hainan Medical University

基　　金：湖北省卫生厅医学科研基金课题(JX2B51)~~

摘　　要：目的:探讨脂多糖(LPS)体外诱导的子宫内膜炎对核因子-κB(NF-κB)信号通路的炎症蛋白和氧化因子的影响。方法:用不同浓度LPS(50、100、200ng/mL)对子宫内膜细胞进行诱导,MTT法检测细胞活力,ELISA试剂盒检测细胞上清白介素-1(IL-1)及前列腺素E2(PGE2)浓度,硫代巴比妥酸法检测丙二醛(MDA)含量,Gries法检测一氧化氮(NO)含量,黄嘌呤氧化法检测超氧化物歧化酶(SOD)活性,western blot检测NF-κB p65的表达及磷酸化情况。结果:随着LPS诱导剂浓度的增加,子宫内膜细胞活力下降,且在48h达到最大抑制程度,细胞上清中SOD活性下降,MDA、NO、IL-1及PGE2含量显著上升,NF-κB p65磷酸化显著。结论:LPS能诱导子宫内膜细胞产生炎症,刺激细胞炎症因子的分泌和抗氧化能力的降低,与NF-κB信号通路有关。Objective： To explored effect of endometritis induced by LPS on level of inflammatory protein and oxidation factor via NF-KB signaling pathway. Methods.. Endometial cell was treated with LPS （50, 100, 200 ng/mL）. The viability of cell was detected by MTT assay. The concentration of IL-6 and PGE2 was tested by elisa method. The concentration of MDA was tested by thiobarbituric acid method. The concentration of SOD was tested by xanthine oxidation method. The concentra- tion of NO was tested by gries method. The expression of NF-KB p65 was assayed by western blot. Results; MTT assay dem- onstrated LPS （50, 100, 00 ng/mL） could suppressed cell viability, and the inhibitory effect was highest in 48 h. With the in- creasing dose of LPS, the activity of SOD decreased, the level of MDA, NO, IL-1 and PGE2 elevated. Finally, LPS stimula- ted NF-KB p65 phosphorylation. Conclusions： These results suggests that endometrial cell treated by LPS can induce inflamma- tiory factor secreted and anti-oxidant ability decreased, which might be related to NF-xB signal pathway.

关 键 词：脂多糖 子宫内膜炎 NF-ΚB信号通路 炎症 氧化 

分 类 号：R711.3[医药卫生—妇产科学]