人单核细胞对煤焦沥青烟提取物诱导人永生化支气管上皮细胞c-Jun mRNA表达的影响  

Effect of Human Acute Monocytic Leukemia Cells on Coal Tar Pitch Extract-Induced c-Jun m RNA Expression in Human Bronchial Epithelial Cells

作　　者：刘文佳[1] 宋金燕[1] 杨维超[1] 晋乐飞 王威[1] 姚武[1] 吴卫东[1] 吴逸明[1] 燕贞[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,河南450001

出　　处：《环境与职业医学》2015年第8期786-789,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:81001240;81001239)

摘　　要：[目的]探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun m RNA表达的影响。[方法]用3μg/m L CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对照组;分别收集各组1、5、10、15、20代细胞。将CC组第9代细胞分别常规培养至10代(CC10组)、15代(CC15组),加入100μg/m L肿瘤坏死因子-α中和抗体培养至15代(中和抗体组)。应用实时荧光定量聚合酶链式反应检测各组细胞中c-Jun m RNA的表达水平。[结果]15、20代CC组人永生化支气管上皮细胞c-Jun m RNA相对表达水平高于CTPE组和二甲基亚砜对照组(均P<0.05);中和抗体组c-Jun m RNA的相对表达水平高于CC10组,低于CC15组(均P<0.05)。[结论]人单核细胞可能通过肿瘤坏死因子-α调控CTPE诱导人永生化支气管上皮细胞c-Jun m RNA的表达。[ Objective ] To explore the effect of human acute monocytic leukemia cells （THP-1） on c-Jun mRNA expression induced by coal tar pitch extract （CPTE） in human bronchial epithelial cells （BEAS-2B）. [ Methods ] BEAS-2B ceils （CTPE group） and co-cultured BEAS-2B and THP-1 cells （CC group） were both simulated by 3 μg/mL CTPE, and dimethyl sulfoxide （DMSO） was used as vehicle control. The cells were collected at passage 1, 5, 10, 15, and 20. The co-cultured cells at passage 9 were further cultured to passage 10 （CC10 group） and passage 15 （CC15 group）, and added 100 μg/mL tumor necrosis factor-or （TNF-ct） neutralizing antibody and further cultured to passage 15 （neutralizing antibody group）. The expression of c-Jun mRNA was detected by real-time fluorescent quantitative polymerase chain reaction. [ Results ] The expression levels of c-Jun mRNA in BEAS-2B ceils at passage 15 and 20 in the CC group were both increased compared with those in the CTPE and DMSO control group （P〈 0.05）. The expression level of c-Jun mRNA after adding TNF-a neutralizing antibody was increased compared with the CC10 group and decreased compared with the CC15 group （P〈0.05）. [ Conclusion ] THP-1 could regulate the expression of c-Jun mRNA induced by CTPE through TNF- α in the BEAS-2B cells.

关 键 词：煤焦沥青烟提取物 C-JUN MRNA 人单核细胞 肿瘤坏死因子-Α 

分 类 号：R114[医药卫生—卫生毒理学]