参芎滴丸对心肌缺血大鼠抗脂质过氧化损伤的保护作用  

PROTECTIVE EFFECT OF SHENXIONG DROP PILLS ON ANTI LIPID PEROXIDATION INJURY IN RATS WITH ACUTE MYOCARDIAL ISCHEMIA

作　　者：杨超燕[1] 吉国辉[1] 何伟[1] 李勇[1] 唐春萍[1] 

机构地区：[1]广东药学院中药学院,广东广州510006

出　　处：《井冈山大学学报（自然科学版）》2015年第4期84-89,共6页Journal of Jinggangshan University (Natural Science)

基　　金：广东省中医药局计划项目(20123012);广州市科技计划项目(2011J4300059)

摘　　要：目的探讨参芎滴丸对异丙肾上腺素诱导大鼠心肌缺血的保护作用及其机制。方法采用异丙肾上腺素(ISO)诱导大鼠急性心肌缺血模型,观察参芎滴丸预给药5 d后对大鼠注射ISO诱导急性心肌缺血后不同时间点心电图(ECG)J点下移的影响;继续给药2 d检测各组大鼠血清中乳酸脱氢酶(LDH)、肌酸磷酸激酶(CK)和天冬氨酸转氨酶(AST)的活性;同时测定大鼠心肌组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量,并观察心肌组织形态学的改变。结果参芎滴丸能明显改善ISO引起的大鼠心电图J点下移变化;降低大鼠血清中LDH、CK、AST的水平和心肌组织中的MDA含量;不同程度地升高心肌组织中的SOD、CAT的活性;明显改善ISO引起的心肌病理损伤;但对GSH-Px活性无明显影响。结论参芎滴丸对大剂量异丙肾上腺素引起的大鼠急性缺血性心肌损伤有明显的保护作用,其机制可能与提高心肌的抗氧化能力、减轻氧化应激反应有关。Objective：To study the protective effect of Shenxiong Drop Pills on the acute myocardial ischemia （AMI） induced by isoprenaline （ISO） and its mechanism.Methods：The AMI rat model was established by injection with ISO. 5 days after administration, the effects of Shenxiong Drop Pills on the electrocardiogram （ECG） at different time points were observed, consecutively administered with Shenxiong Drop Pills for 2 days, the superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, catalase （CAT） and malondialdehyde （MDA） levels of myocardial tissue, lactate dehydrogenase （LDH）, aspartate aminotransferase （AST）, and creatine kinase （CK） activities in serum were detected using biochemical method, and the myocardial tissue pathological histomorphology were observed. Results：Compared with the model group, Shenxiong Drop Pills inhibited the J spot downward of ECG during myocardial ischemia. The levels of LDH and CK in serum and the content ofMDA in myocardial homogenate decreased, the activities of SOD and CAT in the myocardial homogenate increased, and showed less histological change than that in the model control guoup, but the activities of GSH-Px in the myocardial homogenate were not significantly between the model group and Shenxiong Drop Pills groups. Conclusion：Shenxiong Drop Pills has the apparent protective effect on AMI injury, its mechanism may be related with improving the myocardial anti-oxidative ability and decreasing the oxidative stress reaction.

关 键 词：参芎滴丸 心肌缺血 抗氧化 氧化应激 心电图 

分 类 号：R285.5[医药卫生—中药学] R541.6[医药卫生—中医学]