干旱胁迫对番茄幼苗叶片SOD、POD和PAL活性的影响  

Effects of Drought Stress on SOD, POD and PAL Activity in Tomato Seedling Leaves

作　　者：裴冬丽[1] 张红岩[1,2] 张贺[1] 陈瑞[1] 李成伟[3] 

机构地区：[1]商丘师范学院生命科学学院,植物与微生物互作重点实验室,河南商丘476000 [2]商丘师范学院体育学院,河南商丘476000 [3]周口师范学院生命科学学院/植物遗传与分子育种重点实验室,河南周口466001

出　　处：《吉林农业科学》2015年第4期83-86,共4页Journal of　Jilin Agricultural Sciences

基　　金：国家自然科学基金(31071807);河南省教育厅项目(13B210199)

摘　　要：以4个番茄品种为材料,研究了聚乙二醇模拟干旱胁迫处理后,番茄幼苗叶片中SOD、POD和PAL活性的变化。结果表明,4个番茄品种干旱胁迫处理后表现出相同的变化趋势,SOD活性显著增加,于48 h达到最大值,而后下降,整个过程中处理组活性均显著高于对照组;POD活性迅速增加,于24 h达到最大,其中雅致的增幅最大,处理组为对照组的4.13倍,随后活性快速下降,于48 h达到一个低值后,活性又有所回升,整个过程中处理组活性均高于对照组;雅致、Moneymaker处理组PAL活性先升后降,而菜都五号和超级908处理组PAL活性呈缓慢上升,活性均高于对照组。由SOD、POD和PAL活性分别得到的4个番茄品种的胁迫系数高低顺序一致,由高到低依次是雅致、Moneymaker、菜都五号、超级908,说明雅致响应积极,具有相对较强的清除活性氧和修复损伤、适应胁迫环境的能力。Four tomato cultivars used as materials, the change of superoxide dismutase（SOD）, peroxidase（POD）and phenylalanine ammonia-lyase（PAL） activities in tomato seedling leaves under drought stress simulated by poly-ethylene glycol were studied. The results showed that four tomato cultivars had the same tendency. SOD activities in-creased significantly, reached a maximum at 48 h, then, decreased. POD activities increased quickly, reached a max-imum at 24 h, and Yazhi had the highest increase which activities of treatment group was 4.13 times of control group.Then, the activities decreased quickly, after reaching a low value at 48 h, it increased to some extent. In the wholeprogress the POD activities of treatment group were higher than the control group. The PAL activities of Yazhi andMoneymaker in treatment group increased at first, then, decreased. The activities of Caidu No. 5 and Super 908 in-creased slowly, and the PAL activities of treatment group were higher than the control. The orders of stress coeffi-cient obtained from SOD activity, POD activity and PAL activity respectively were identical. The stress coefficientranked from high to low was Yazhi, Moneymaker, Caidu No. 5, Super 908, indicating that Yazhi responded activelyand had relatively strong ability of removing reactive oxygen, repairing the damage and adapting to the drought stress.

关 键 词：聚乙二醇 干旱胁迫 超氧化物歧化酶 过氧化物酶 苯丙氨酸解氨酶 

分 类 号：S641.2[农业科学—蔬菜学]