再力花生物湿地床氮磷去除过程中根系微生物群落结构解析  

Analysis of Microbial Community Structure in The Process of Nitrogen and Phosphorus Removal in Thalia Dealbata Bio-Rack Wetland

作　　者：刘勇波[1] 王骥[1] 张兰英[1] 

机构地区：[1]地下水资源与环境教育部重点实验室/水资源与水环境吉林省重点实验室/吉林大学环境与资源学院,长春130021

出　　处：《吉林农业科学》2015年第4期104-107,共4页Journal of　Jilin Agricultural Sciences

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07101-012009ZX07101-09);国家高技术研究发展计划项目(2005AA60101005)

摘　　要：生物湿地床作为富营养化水体生态修复技术的重要组成部分,具有良好的发展潜能,其应用的关键是优选适宜的植物种类,并维持较高的污水净化能力。目前,国内外对湿地床根系微生物的群落结构与优势种群组成及变化研究甚少。本文利用变性梯度凝胶电泳技术(DGGE)对再力花湿地床不同根系微生物群落结构进行了解析。结果表明,腐根和黄根间具有较高相似度种群,而黄根、腐根与幼根相似度较低。微生物种类在腐根根系最为丰富,幼根最少。Clavibacter、Clostridium、Mesoflavibacter、Brevundimonas、Chromobacterium在生物湿地床去除污染物的过程中起到重要作用,为主要功能菌群。研究结果对应用生物湿地床净化水体中氮、磷具有一定的参考价值和理论意义。As an important part of ecological remediation technology for eutrophication water, bio-rack wetland has a good development potential, and the keys to its application are selecting the appropriate plant species and maintaining a high sewage purification capacity. At present, there are few researches on microbial community structure and composition and changes of dominant population in root system of bio-rack wetland. In this paper, the mi-crobial diversity of different roots in Thalia dealbata bio-rack wetland was analyzed through PCR-DGGE. The re-sults showed that, rotted roots and yellow root had a high similarity in microbial populations, while the young rootand yellow root, rotted roots had a low similarity. The microbial species of rotted roots were abundant, but relativelyyoung root had few. Clavibacter sp., Clostridium sp., Mesoflavibacter, Brevundimonas sp. and Chromobacterium sp.played an important role in the removal of contaminant for bio-rack wetland. The research results have a certain reference value and theoretical significance for the application of bio-rack wetland to purify the water body.

关 键 词：再力花 湿地床 微生物 多样性 

分 类 号：S154[农业科学—土壤学]