1型糖尿病小鼠胰岛微血管内皮细胞超微结构受损  

Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice

作　　者：张旭[1] 刘明明[2] 程明[1] 徐进[1] 王书合[1] 王惠[1] 任雅丽[1] 修瑞娟[2] 王素霞[1] 

机构地区：[1]北京大学第一医院电镜室,超微病理中心,北京100034 [2]中国医学科学院北京协和医学院微循环研究所,卫生部微循环重点实验室,北京100005

出　　处：《中国病理生理杂志》2015年第8期1520-1524,1530,共6页Chinese Journal of Pathophysiology

摘　　要：目的:探讨1型糖尿病小鼠胰岛微血管内皮细胞的超微结构改变。方法:BALB/c小鼠随机分为糖尿病组和对照组,每组6只。应用免疫组化染色检测胰岛素及胰岛微血管血小板内皮细胞黏附分子-1(CD31)表达水平;应用透射电镜观察糖尿病小鼠胰岛β细胞及胰岛微血管超微结构变化。结果:糖尿病组小鼠胰岛β细胞数量、β细胞/α细胞数量比、β细胞分泌颗粒数量及胰岛素阳性染色面积百分比显著降低(P<0.01)。糖尿病组小鼠胰岛微血管数量,CD31表达水平显著降低(P<0.01);微血管内皮细胞及胰岛周细胞线粒体肿胀变性;微血管基膜厚度显著升高(P<0.01)。结论:1型糖尿病小鼠胰岛微血管内皮细胞超微结构受损。AIM： To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS： BALB / c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1（ CD31） in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS： Compared with control group,the number of islet β cells,ratio of β cells / α cells,average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased（P〈0. 01）. Besides,diabetic group had fewer microvessels with lower expression of CD31（P〈0. 01）. Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group（P〈0. 01）. CONCLUSION： Islet microvascular endothelial cells were impaired in type 1 diabetic mice.

关 键 词：胰岛微血管内皮细胞 超微结构 1型糖尿病 胰岛微循环 

分 类 号：R587.1[医药卫生—内分泌]