玉米茉莉酸甲酯不敏感突变体的筛选  

Screening of Methyl Jasmonate-Insensitive Mutants in Maize

作　　者：查象敏 汪海[1] 路小铎[2] 张春义[1] 黄大昉[1] 郎志宏[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081 [2]齐鲁师范学院生命科学学院,山东章丘250200

出　　处：《作物学报》2015年第9期1454-1461,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31270317)资助

摘　　要：植物激素茉莉酸(jasmonic acid,JA)作为一种可以长距离运动的信号分子在植物对昆虫侵害的系统抗性中起核心调控作用。为了解析玉米JA信号途径及抗虫机制,本研究利用改进的玉米突变体的筛选方法,筛选与JA信号途径相关的突变体用于下一步的研究。将甲基磺酸乙酯(ethyl methane sulfonate,EMS)诱变的玉米突变体库6118份种子分别置含有外源茉莉酸甲酯(methyl jasmonate,MeJA)的培养基中培养,通过观察玉米根系的生长情况,确定对MeJA的不同耐受性。当MeJA终浓度为50μmol L–1时,初步筛选到对MeJA不敏感的I级耐受性突变体61株;当MeJA终浓度分别为100μmol L–1和200μmol L–1时进行验证,得到的耐受性事件分别为37株和10株。对自交收获的子一代遗传性分析表明,这些突变体对MeJA表现出的耐受性具有可遗传性。筛选获得的对MeJA不敏感突变体可能与JA调控有关,是研究玉米JA信号途径的很好材料。The phytohormone jasmonate plays a pivotal role in plant systematic resistance to herbivores attack as a long-distance mobile signal. To demonstrate the Jasmonic acid （JA） signal pathway and the insect-resistant mechanism in maize, we utilized an improved approach to screen the maize mutants associated with JA signal pathway, which will be available for further research. The 6118 lines＇ seeds from EMS-mutagenic maize mutants library were cultivated in the medium containing the exogenous MeJA The different tolerances of the mutants to MeJA were identified by observing the growth of maize roots. When the final concentration of MeJA was 50 μmol· L^-1, 61 MeJA-insensitive mutants with tolerance Grade I were obtained in the primary screening experiment. When the final concentration of MeJA was 100 μmol· L^-1 or 200 μmol· L^-1, 37 and 10 tolerant events were gained in the confirmation experiments, respectively. Then, we analyzed the heredity of M 1 seeds tolerant to MeJA. The results showed that the tolerance of the mutants to MeJA was hereditable. The MeJA-insensitive mutants associated with JA regulation pathway are the potential materials for the further studies of JA signal pathway and insect resistant mechanism in maize.

关 键 词：MeJA/JA 玉米突变体库筛选 MeJA不敏感 玉米虫害应答 

分 类 号：S513[农业科学—作物学]