全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制  

Quality control of adding sample on HBcAb by automatic enzyme immunoassay instrument

作　　者：张龙[1] 银广悦[1] 武彩虹[1] 王辉[2] 张朝云[3] 

机构地区：[1]中国石油中心医院检验科,河北廊坊065000 [2]中国石油中心医院急诊科,河北廊坊065000 [3]中国石油中心医院内分泌科,河北廊坊065000

出　　处：《检验医学与临床》2015年第16期2342-2343,共2页Laboratory Medicine and Clinic

基　　金：河北省廊坊市科技局项目(2013013020C)

摘　　要：目的选择TECAN全自动酶联免疫分析仪检测乙肝核心抗体(HBcAb)的理想检测程序。方法对同一样本设置不同的加样方式、加样顺序,获得相应的数据,比较检测结果以确定理想检测程序。结果 TECAN全自动酶联免疫分析仪检测HBcAb,加样针存在拖带现象,可能造成标本污染,出现假阳性结果,或污染阴性对照,使CO值降低,造成假阴性结果。通过改进加样程序,减少携带概率,避免阴性对照污染,提高检测质量。结论用单独加样方式,先加阴阳对照再加标本的顺序为理想的检测程序。Objective To select the ideal examination program of the TECAN automatic enzyme immunoassay instrument for detecting HBcAb. Methods The different adding modes and adding sequences were set on the same sample for obtaining the corresponding data. Results In detecting HBcAb by the TECAN automatic enzyme immu- noassay instrument, adding sample needle had the entrainment phenomenon, which could cause the sample contamina- tion,thus led to false positive results or contaminated the negative control to make the CO value decrease and cause the false negative result. The improvement of adding sample program reduced the carrying probability, avoided the negative control pollution and increased the detection quality. Conclusion Adopting the single adding sample mode, and first adding negative and positive control and then sample are the ideal detection program.

关 键 词：乙肝核心抗体 全自动酶联免疫分析仪 质量控制 

分 类 号：R446[医药卫生—诊断学]