心脑舒通对缺氧/复氧损伤神经细胞的保护作用及其机制研究  

Protective effect of Xinnao Shutong on hypoxia / reoxygenation injured nerve cells and its mechanism

作　　者：马萌萌[1] 胡利民[1] 王乔悦 杨红云[1] 王旭梅[1] 郭虹[1] 

机构地区：[1]天津中医药大学中医药研究院天津市现代中药重点实验室--省部共建国家重点实验室培育基地天津市中药药理学重点实验室,天津300193

出　　处：《天津中医药大学学报》2015年第4期245-247,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家科技重大专项项目-重大新药创制(2011ZX09201);"十二五"期间天津市高等学校"创新团队培养计划"(TD12-5035)

摘　　要：[目的]研究心脑舒通对缺氧/复氧损伤神经细胞的保护作用及其机制。[方法]体外培养Neuro-2a细胞,分为正常对照组、缺氧/复氧损伤模型组、心脑舒通(10、25、50 mg/L)给药组,建立缺氧缺糖(OGD)4 h/复氧复糖(Rep)24 h损伤模型,检测心脑舒通对各组细胞增殖活力、乳酸脱氢酶(LDH)漏出量、活性氧(ROS)水平和线粒体膜电位(△Ψm)的影响。[结果]与缺氧/复氧组比较,心脑舒通可以明显增加Neuro-2a细胞活力,减少LDH漏出,降低ROS水平,增强线粒体膜电位。[结论]心脑舒通对缺氧/复氧损伤神经细胞具有明显保护作用,其机制与其影响活性氧水平和线粒体膜电位有关。[Objective]To study the protective effect of Xinnao Shutong on hypoxia / reoxygenation injured nerve cells and its mechanism. [Methods] Neuro-2a cells were cultured in vitro, divided into control, model, low-dose, mid-dose and high-dose Xinnao Shutong （10,25、50 g/mL） groups. Establishmenting OGD 4 h/Rep 24 h damage model, detecting cell viability, lactate dehydrogenase （LDH） leakage ,reactive oxygen species （ROS）level and mitochondrial membrane potential （A ψm）. [Results] Compared with model group Xinnao Shutong can significantly increase the Neuro-2a cell activity, reduce the leakage of LDH, decrease the level of ROS, enhance the mitochondrial membrane potential. [Conclusion] Xinnao Shutong has obvious protective effect on hypoxia/reoxygenation injured nerve cells, and the mechanism is related to influence ROS level and mitochondrial membrane potential on Neuro-2a.

关 键 词：心脑舒通 神经保护 缺氧 复氧 Neuro-2a细胞 

分 类 号：R285.5[医药卫生—中药学]