亚甲蓝光化学法灭活对血浆HBV前S2基因降解的研究  

Study on the degradation of hepatitis B virus S2 gene in plasma by methylene blue photochemical method

作　　者：曹鸿霖 黎淦平 涂传清[3] 刘宜仲 张爱芬 曾凌晓 

机构地区：[1]深圳市宝安区中心血站,广东深圳518000 [2]深圳市宝安区中医院 [3]深圳市宝安区人民医院

出　　处：《中国输血杂志》2015年第7期787-788,共2页Chinese Journal of Blood Transfusion

摘　　要：目的探讨亚甲蓝处理HBV血浆前S 2抗原(HBV Pre S2-Ag)与其DNA降解的关系。方法将乙肝表面抗原和前S2抗原均阳性的血浆,分为未处理组,亚甲蓝处理0、5、10、20、35 min,共6组,每组20 m L。ELISA检测处理前后pres2蛋白变化;设计多对引物,含重叠区,检测pres2基因DNA完整性。结果亚甲蓝能降解DNA序列,并且呈现区域特异性。结论亚甲蓝在基因水平灭活病毒,并且呈现核酸序列特异性,对pres2蛋白含量没有影响。运用核酸检测技术,对评价血浆HBVpres2基因病毒灭活效果的有一定的意义。Objective To investigate the relationship between HBV S2（ Pre S2-A g DNA） and the degradation of HBV in methylene blue. Methods The hepatitis B surface antigen and S2 antigen were positive in plasma,which were divided into untreated group and methylene blue was treated with 0,5,10,20,35 min,a total of 6 groups,each group 20 m L. The change of pre S2 protein was detected by ELISA,and the DNA gene was detected by pre S2. Results methylene blue can degrade the DNA sequence,and it has a regional specificity. Conclusion Methylene blue at the gene level of inactivated virus,and the specificity of nucleic acid sequence,the content of pre S2 protein did not affect. Using nucleic acid detection technology,it has a certain significance to the evaluation of the effect of plasma HBV pres2 gene virus inactivation.

关 键 词：亚甲蓝光化学法 灭活 HBV前S2蛋白 

分 类 号：R512.62[医药卫生—内科学] R457.14[医药卫生—临床医学]