镉对苗期高羊茅的形态和生理影响  被引量:7

Effects of heavy metal cadmium on morphology and physiology of Festuca arundinacea seedlings

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作  者:范金[1] 袁庆华[1] 

机构地区:[1]中国农业科学院北京畜牧兽医研究所,北京100193

出  处:《草业科学》2015年第8期1278-1288,共11页Pratacultural Science

基  金:国家十二五支撑项目-优良多抗牧草新品种选育及良种繁育关键技术研究与示范(2011BAD17B01)

摘  要:以20份高羊茅(Festuca arundinacea)品种为试验材料,通过测量其形态指标,运用标准差系数赋予权重法,对其进行综合评价。结果筛选出耐镉性较强的高羊茅材料M15和M8,分别采自美国的猎狗5和荷兰的巴弗斯;耐镉性较差的高羊茅材料M5和M11,分别采自荷兰的米斯特拉尔和来自日本的法恩。在此基础上,采用分光光度法,测定了这4份材料的生理指标,过氧化物酶(POD)、过氧化氢酶(CAT)、叶绿素和脯氨酸(Pro)含量的变化,进一步研究了他们的生理反应。结果表明,随着镉浓度的升高,耐镉性较强的高羊茅材料猎狗5与和巴弗斯POD和CAT含量一直呈上升趋势,而耐镉性较差的材料米斯特拉尔和法恩POD和CAT含量则呈现先升后降的趋势。随着镉浓度的升高,不同材料的叶绿素含量呈下降趋势,不同材料的脯氨酸含量则呈不断上升趋势。A comprehensive evaluation of 20 Festuca arundinacea cultivars tolerance to heavy metal cadmium( Cd)was conducted by measuring their morphological and physiological response under different Cd2 +concentrations.The results showed that survival rate,plant height,above ground biomass and leaf water content significantly decreased with the cadmium concentration increasing whereas tiller numbers firstly increased and then decreased with the cadmium concentration increasing. Two strong cadmium tolerant cultivars( M15 and M8) and two weak cadmium tolerant cultivars( M5 and M11) were screened using the standard deviation coefficient allocation weight method.And then,the physiological response of these 4 cultivars to Cd2 +including the activities of peroxidase( POD) and catalase( CAT) and the contents of chlorophyll and proline( Pro) were measured. With the cadmium concentration increasing,the activities of POD and CAT of 2 strong cadmium tolerant cultivars increased whereas those of 2 weak cadmium tolerant cultivars firstly increased and then decreased. With the cadmium concentration increasing,the chlorophyll contents of all tested cultivars decreased and Pro contents of all tested cultivars increased.

关 键 词:高羊茅 重金属镉 生理指标 综合评价 

分 类 号:Q945.78[生物学—植物学]

 

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