HPLC法测定骨刺片中柚皮苷、芍药苷的含量  

Determination of Naringin and Paeoniflorin in Gu CI Tablet by HPLC

作　　者：张舒[1] 袁晓芳[1] 张艳[1] 

机构地区：[1]淮安市食品药品检验所,江苏淮安223300

出　　处：《广州化工》2015年第16期140-142,共3页GuangZhou Chemical Industry

摘　　要：测定骨刺片中柚皮苷、芍药苷的含量。采用HPLC法测定柚皮苷、芍药苷的含量。色谱柱：Inertsil ODS-SP C18（250mm×4．60mm，5μm）；检测波长：283nm；流动相：乙腈-0．1％醋酸溶液梯度洗脱；柱温30℃，流速0．8mL／min；进样量：10μL·mL^-1。柚皮苷：线性范围为35．19μg·mL^-1～-1．7595mg·mL^-1，r=0．99990，平均回收率为102．50％，RSD为0．5％（n=6）。芍药苷：线性范围为35．74}zg·mL^-1～-1．7870mg·mL^-1，r=0．99990，平均回收率为99．79％，RSD为0．9％（n=6）。该方法简便、快速、准确，为提高和完善该制剂的质量标准提供了依据。To determine the content of Naringin and Paeoniflorin in Gu CI Tablet, Naringin and Paeoniflorin were determined on a Inertsil ODS-SP C1s（250 mm×4.60 mm, 5 μm） column used with the mobile phase of acetonitrile- 0. 1% acetic acid solution with gradient elution, 283 nm as the detection wavelength, 30℃ as the column temperature and 0. 8 mL · min-1 as the flow rate. Result showed that the linear range of Naringin was 35. 35 μg · mL-1 - 1. 7675 mg · mL-1 （r = 0. 99990）. The average recovery was 102. 50% （ RSD = O. 5% , n = 6）. The linear range of Paeoniflorin was 36. 19μg · mL-1 - 1. 8095 mg · mL-1 （ r = 0. 99990）. The average recovery was 99.79% （ RSD = 0. 9%, n = 6）. The method is simple, accurate and repeatable, and can provide the basis for the improvement and optimization of the quality standard of Gu CI Tablet.

关 键 词：高效液相色谱法 骨刺片 柚皮苷 芍药苷 含量测定 

分 类 号：R28[医药卫生—中药学]