稻瘟病抗性基因Pi9的功能标记的开发  

Development of Functional Marker on the Rice Blast Resistance Gene Pi9

作　　者：张羽[1] 李厚华 王保军 李小刚 周凯 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000 [2]陕西省水稻研究所,陕西汉中723000

出　　处：《云南农业大学学报（自然科学版）》2015年第4期528-534,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：陕西省科技厅农业科技攻关项目(2013K02-10-01);陕西省科技厅农业科技创新项目(NKC01-01);陕西省科技统筹创新工程计划项目(2011KTCG02-01)

摘　　要：Pi9基因来源于小粒野生稻,是迄今为止已报道的83个稻瘟病主效抗性基因之一,对稻瘟病具有广谱抗性。对‘WHD75-1-127’的Pi9基因序列和感病对照材料‘Nipponbare’及‘93-11’进行同源性比较,根据不同Pi9基因型水稻的序列差异,构建与Pi9基因紧密连锁的SNP共显性标记,对试验条件进行了探索优化,并与已经报道的显性标记PB8和共显性标记PB9-1比较。结果显示:在Pi9基因区域开发的SNP标记比已经报道的Pi9基因标记简单、有效、准确。Pi9 gene derives from Oryza minuta and is one of the mare eitect resistance ～n～ blast in the reported 83 genes. The homologous sequences of Pi9 gene, ＇ Nipponbare＇ and ＇ 93-11＇ in rice were compared. Based on the sequence difference of Pi9 genotypes, the SNP co-dominant marker closely chain to Pi9 was developed and compared with previously reported dominant marker PB8 and co-dominant markers PBg-1 ; the experimental condition was optimized as well. The results demonstra- ted that SNP markers developed in regions of Pi9 gene in this study were much more simple, effective and accurate than PB8 and PB9-1 markers.

关 键 词：稻瘟病 Pi9基因 功能标记 开发 

分 类 号：S332[农业科学—作物遗传育种]