防治软腐病的魔芋内生细菌的筛选与鉴定  

The Screening and Identification of Endophytic Bacteria of Amorphophallus konjac against Erwinia carotovora var. carotovora

作　　者：周丽洪[1,2] 李淼[1] 姬广海[1] 王永吉[1] 杨俊[1] 魏兰芳[3] 

机构地区：[1]云南农业大学,云南省农业生物多样性利用与保护重点实验室,云南昆明650201 [2]四川理工学院,生物工程学院,四川自贡643000 [3]云南农业大学农科基础实验教学中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2015年第4期547-553,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：农业部公益性行业专项(201303015);国家自然基金(31360002,31460458)

摘　　要：本研究采用相似生境分离方法分离魔芋内生细菌后经室内平板初筛、温室复筛试验筛选获得对魔芋软腐病具有良好防治效果的生防菌。研究共分离出127株内生细菌,经过多重筛选后获得3株抑菌效果较好的生防菌(M3,M2,1-7)。经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和16S r DNA序列分析等方法,将M3,M2,1-7分别鉴定为:枯草芽孢杆菌(Bacillus subtilis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和成团泛生菌(Pantoea agglomerans)。这是将寄主植物内生细菌作为生防菌防治该病害的首次报道。In this study, 127 endophytic bacteria of konjac were isolated by similar habitat sepa- ration method. Three strains （M3, M2, 1-7 ） were screened out for control the soft rot of Amor- phophallus konjac by plate screening test and greenhouse rescreening test. The bacteria strains M3, M2, 1-7 were identified as Bacilus subtilis, Bacillus amyloliquefaciens and Pantoea agglom- erans respectively, according to the characters of cultural colonies, morphological observation, biochemical and physiological identification （Biolog carbon source utilization analysis） , 16S rDNA sequences analysis. This is the first report of use host plant endophytic bacteria as biocontrol a- gents to control the disease.

关 键 词：魔芋内生细菌 魔芋细菌性软腐病 生物防治 鉴定 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]