CD11c+DC和CD103+DC及CD68+Mφ在双峰驼肠系膜淋巴结中的分布特点  

Distribution characteristics of CD11c+DC,CD103+DC and CD68+Mφ in the mesenteric lymph nodes of Bactrian camel

作　　者：李淑娴[1] 郭明刚[1] 张学锋[1] 曹婷婷[1] 刘磊[1] 范希萍[1] 王雯慧[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医科学》2015年第8期843-847,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31260595;30671549);教育部博士点基金项目(200807330004)

摘　　要：为了探讨CD11c和CD103分子标记物标记的树突状细胞(DC)及CD68分子标记物标记的巨噬细胞(Mφ)在双峰驼肠系膜淋巴结(MLN)中的形态特点和分布规律,采用SABC免疫组织化学技术对10峰成年双峰驼MLN不同部位的DC和Mφ进行了定位和观察。结果显示,CD11c阳性树突状细胞(CD11c+DC)的核较大淡染,突起细长且弯曲,环抱周围的淋巴细胞,主要分布在生发中心、滤泡间区和小梁周围;CD103阳性树突状细胞(CD103+DC)的形态在不同部位相似,细胞核大、淡染,突起不明显,形态规则,主要分布在小梁周围和滤泡间区,髓质也有较多分布,滤泡生发中心分布较少。结果表明,CD11c+DC和CD103+DC在MLN中的主要分布差异是,前者在生发中心分布较多,而后者在髓质分布较多。CD68+Mφ主要分布于胸腺依赖区的高内皮毛细血管后微静脉和髓质淋巴窦。上述三种细胞的分布特点均与它们的抗原递呈功能密切相关。To explore the morphological characteristics and distribution laws of dendritic cells marked by Integrin alpha-E（CDllc） or Integrin alpha-X（CD103） and macrophage marked by CD68＋Mφ in the mesenteric lymph nodes of Bactrian camel, we located and observed DC and Mφ distributing in the different positions of the mesenteric lymph nodes from 10 adult Bactrian camels by the SABC immunohistochemistry technique. In result, the CD11c＋DC＇s nucleus were large, slightly stained and the protuberance was long and thin, surrounding the peripheral lymphocytes. These cells mainly distributed in germinal center,the interfollicular areas and around the trabecula. The cell morphology of the CD103＋DC in different places was similar, with large nucleus, slightly stained and less obvious protuberance, regular cells morphology. They were mainly distributed in the interfollicular areas and around the trabecula as well as distributed extensively in the medulla, but distributed relatively less in the germinal center. The results indicated that the distribution of CD11c＋DC and CD103 ＋DC in mesenteric lymph nodes varies considerably. The former distributed more in the germinal center and the latter distributed more in the medulla. CD68＋Mφ mainly distributed in the HEV of thymus dependent area and medullary sinus. The distribution characteristics of the three cells related closely with their functions for antigen presenting.

关 键 词：双峰驼 肠系膜淋巴结 CD103+DC CD11c+DC CD68+Mφ 

分 类 号：S852.42[农业科学—基础兽医学]