蛹虫草多糖对小鼠脾淋巴细胞增殖及IL-2和IFN-γ分泌的影响  

Effects of Cordyceps militaris polysaccharides on proliferation and induction of cytokines of splenic lymphocytes in mouse

作　　者：王米[1] 张丽芳[1] 杨锐乐[1] 孟新宇[1] 费陈忠[1] 张可煜[1] 王霄阳[1] 郑文丽[1] 王春梅[1] 薛飞群[1] 

机构地区：[1]中国农业科学院上海兽医研究所动物药学研究室,上海200241

出　　处：《中国兽医科学》2015年第8期848-853,共6页Chinese Veterinary Science

基　　金：公益性行业(农业)科研专项(201303040-08);中央级公益性科研院所基本科研业务费专项(2010JB14);国家高技术研究发展计划(863)项目(2011AA2114);国家自然科学基金项目(31272607)

摘　　要：为探讨蛹虫草多糖（CMP）的免疫活性,将6种浓度的CMP40和CMP50分别单独或与Con A、LPS混合加入到小鼠脾淋巴细胞的培养体系中,培养后第48小时,采用MTT法测定淋巴细胞增殖（D570nm值）的变化;将5种浓度的CMP40和CMP50分别与Con A加入到小鼠脾淋巴细胞的培养体系中,培养24h后收集细胞培养上清液,测定IL-2和IFN-γ的质量浓度。结果显示,多糖浓度在62.5~500μg/mL时,CMP40＋Con A组和CMP50＋Con A组的D570nm值均显著高于Con A对照组,CMP50＋LPS组的D570nm值显著高于LPS对照组;多糖浓度在31.25μg/mL时,CMP40＋LPS组的D570nm值显著高于LPS对照组;CMP40在31.25~500μg/mL组、CMP50在250μg/mL组的D570nm值显著高于细胞对照组。淋巴细胞增殖率结果表明,CMP50协同Con A或LPS刺激淋巴细胞增殖的效果强于CMP40。CMP40和CMP50在500μg/mL组的IL-2浓度显著高于对照组,CMP40在500μg/mL组和CMP50在62.5~125μg/mL组的IFN-γ浓度显著高于对照组。结果表明,CMP40和CMP50在合适剂量下能单独或协同Con A、LPS刺激T、B淋巴细胞增殖,促进淋巴细胞分泌IL-2和IFN-γ,从而增强细胞免疫。To investigate effects of Cordyceps militaris polysaccharides（CMP） on immune activity, six concentrations of CMP40 and CMPs0 sole and/or with Con A, LPS were added to the culture system of mouse spleen lymphocytes for 48 h,respectively,and lymphocyte proliferation（D570 nm） changes were determined by MTT method. Five kinds of concentrations of CMP40 and CMP50 with Con A were added to the culture system of mouse spleen lymphocytes, respectively. The concentration of IL-2 and IFN-γ in the supernatant of cell culture harvested after 24 h was determined. The results showed that the D570nmvalues of CMP40 ＋Con A and CMP50 ＋Con A group were significantly higher than that at Con A control group when the concentration of polysaccharide range from 62.5 to 500 μg/mL, the D570 nm values of CMP50 ＋ LPS group was significantly higher than that at LPS group when the concentration polysaccharide at 31.25μg/mL/the D570 nm values of CMP40＋LPS group was significantly higher than that at the LPS control group when the concentration,CMP40 range from 31.25 to 500 μg/mL group, and CMP50 at 250μg/mL group; the D570nm values were higher than the cell control group. Lymphocyte proliferation rates of CMP40 joint with Con A or LPS were stronger than CMP50. In CMP40 and CMP50 at 500 μg/mL group,IL-2 concentrations were significantly higher than those at control groups. In CMP40 at 500μg/mL and CMP50 at 62.5 to 125μg/mL group,the concentrations of IFN-γ were significantly higher than those at control groups. The results indicated that CMP40 and CMPs0 at suitable doses can solely or jointly with Con A or LPS stimulate T and B lymphocyte proliferation, and promote the lymphocyte secretion of IL-2 and IFN-γ,thereby enhancing the cellular immunity.

关 键 词：多糖 小鼠 脾淋巴细胞 增殖 白介素-2 Γ-干扰素 

分 类 号：S853.7[农业科学—临床兽医学]