Wnt3a基因沉默对内皮祖细胞增殖的影响  

Effects of wnt3a silencing on the proliferation of endothelial progenitor cells

作　　者：余涛[1] 杜怡斌[1] 杜公文 张辉[1] 张硕[1] 方家刘 高维陆[1] 尹宗生[1] 

机构地区：[1]安徽医科大学第一附属医院骨科,合肥230022

出　　处：《安徽医科大学学报》2015年第9期1211-1214,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81171173);安徽省自然科学基金(编号:11040606Q25)

摘　　要：目的探讨wnt3a基因沉默对内皮祖细胞(EPCs)增殖的影响。方法体外分离、培养及鉴定EPCs。将沉默型p EGFP-shRNA-wnt3a重组质粒转染EPCs;Western blot法分析wnt3a在EPCs中的表达变化;MTT分析wnt3a沉默对EPCs增殖的影响。结果鉴定培养的细胞为EPCs;Western blot法结果提示p EGFP-shRNA-wnt3a转染EPCs后明显抑制了wnt3a蛋白的表达。MTT提示沉默型pEGFP-shRNAwnt3a转染组吸光度值明显低于空白对照组。结论成功分离、培养出EPCs,wnt3a有效沉默明显抑制EPCs的增殖,为进一步的实验提供了基础。Objective To observe the effects of wnt3a silencing on the proliferation of endothelial progenitor cells （EPCs）. Methods We used the mature methods of isolation, culture and identification on cells to get EPCs in vitro. The endothelial progenitor cells were transfeeted with the the silent type pEGFP-shRNA-wnt3a recombinant plasmid. The expression changes of wnt3a were detected by Western blot. MTI＇ assay was used to assess the effects of wnt3a silencing on the proliferation of EPCs. Results The cultured cells were confirmed to be EPCs. The results of Western blot revealed that wnt3a expression was obviously inhibited in transfected EPCs with pEGFP-shR- NA-wnt3a. MTr assay showed that the proliferation of EPCs was significantly inhibited by effective silencing of wnt3a as compared with the control group. Conclusion We have successfully isolated and cultured EPCs in vitro. Wnt3a effectively silence inhibits the proliferation of EPCs. And this study provides the basis for further experi- ments.

关 键 词：内皮祖细胞 WNT3A 基因沉默 基因转染 细胞增殖 

分 类 号：R681.54[医药卫生—骨科学]