白芍总苷对原发性干燥综合征浆细胞样树突状细胞影响的研究  

Total glucosides of paeony attenuated activation of plasmacytoid dendritic cells in primary Sjgren's syndrome patients

作　　者：李晓玲[1] 汪国生[1] 李向培[1] 厉小梅[1] 

机构地区：[1]安徽医科大学附属省立医院风湿免疫科,合肥230001

出　　处：《安徽医科大学学报》2015年第9期1281-1285,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81072462)

摘　　要：目的探讨白芍总苷(TGP)治疗对原发性干燥综合征(pSS)患者外周血浆细胞样树突状细胞(pDC)的影响。方法收集新发pSS患者17例作为疾病组,每日予以TGP 1.8g治疗3个月,对照组17例。采用流式细胞术检测疾病组治疗前、后及对照组pDC及其表面CD32、CD86、CXCR4和Blys表达;ELISA法检测血浆干扰素-α(IFN-α)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;免疫组化法检测25例pSS患者唇腺pDC浸润。结果 1 pSS患者外周血pDC比例较对照组下降,pDC表达CXCR4比例较对照组增加,血浆IL-6、TNF-α、IFN-α水平较对照组上升,25例pSS患者唇腺中19例有pDC浸润;2 TGP治疗后,pSS患者外周血pDC比例较治疗前上升;pDC表达CXCR4、Blys比例较治疗前下降;TGP治疗后血沉下降;血浆IL-6、TNF-α水平治疗后下降。结论新发pSS患者较正常者外周血pDC比例降低,表达CXCR4增加,唇腺组织中有pDC浸润;TGP可能抑制pSS患者pDC活化。Objective To investigate the effect of TGP on pDCs in new pSS patients and its mechanism of anti-inflammatory effects. Methods 17 new pSS without any treatment were recruited as disease group, accepted total glucosides of paeony for 3 months, and 17 healthy subjects as control group. The percentages of peripheral blood pDCs, circulating plasma IFN-α, IL-6, TNF-α levels were analysed by Flow cytometry and ELISA. Immunohisto- chemistry method was used to observe the pDCs infiltration in labial gland. Results New pSS patients had signifi- cantly lesser pDCs in PB, and CXCR4 expressed by pDCs were higher than healthy controls. TGP could inhibit pDC activation and then reduce CXCR4 expression. Plasma IFN-α, IL-6, TNF-α levels of new pSS were increased, and TGP reduced IL-6,TNF-α levels. Moreover, pDCs were present in labial gland of pSS under the effect of chemo- kines receptor CXCR4. Conclusion The findings reveal that activated pDCs transfer to the labial gland under the effect of chemokines receptor CXCR4. Total glucosides of paeony inhibit the activation of pDCs in primary Sjsgren＇ s syndrome.

关 键 词：原发性干燥综合征 白芍总苷 浆细胞样树突状细胞 TNF-Α IFN-Α IL-6 

分 类 号：R392.5[医药卫生—免疫学]