金双歧肠内免疫生态营养对创伤后大鼠肠道免疫功能及损失修复的影响  

Effects of golden bifid intestinal immune ecological supplements on rat intestinal immune and its damage repair

作　　者：李诗杰[1] 栗芳军[1] 杨志家[1] 郭俊强[1] 王勇[1] 

机构地区：[1]河南大学淮河医院急诊创伤科,河南开封457000

出　　处：《中国临床药理学杂志》2015年第16期1629-1631,共3页The Chinese Journal of Clinical Pharmacology

基　　金：河南省教育厅科学技术研究重点基金资助项目(142102310527)

摘　　要：目的探讨金双歧肠内免疫生态营养制剂对大鼠肠道免疫及损伤修复功能的影响。方法雄性清洁级Wistar大鼠30只,小肠损伤造模成功后,按体重随机分为对照组15只和实验组15只。造模成功后12 h,2组开始分4次给予180 kcal·(kg·d)-1的标准等氮热量灌胃,实验组同时给予金双歧10 CFU·d-1,灌胃7 d后,断颈处死大鼠。取大鼠小肠组织,苏木素-伊红染色,观察小肠绒毛高度、肠腺隐窝深度;用免疫组织化学技术检测小肠组织中免疫球蛋白A(Ig A)、及CD3+阳性T淋巴细胞、CD4+阳性T淋巴细胞、CD8+阳性T淋巴细胞表达水平,比较2组的差别。结果对照组,大鼠体重增长速度低于实验组,第5天开始,2组大鼠体重差别有统计学意义(P<0.05);实验组,大鼠小肠黏膜绒毛高度、腺隐窝深度和黏膜厚度均显著高于对照组(均P<0.05);实验组的Ig A、CD3+、CD4+及CD8+阳性淋巴细胞数均明显多于对照组(P<0.05)。结论肠内免疫生态营可增强大鼠肠道免疫功能,促进损伤修复。Objective To explore the efficacy of live combined bifidobacterium and lactobacillus tablets（ Jinshuangqi） enteral ccological immune nutrition on the intestinal immune and its damage repair in wound Wistar rats. Methods Thirty male Wistar rats of small intestine trauma model were divided into two groups： experiment and control groups with each 15 rats. Rats in the control group were given regular nutrition forage and rats in the experiment group were given live combined bifidobacterium and lactobacillus tablets with 10 CFU·d^- 1. At time of 7days after administration the rates were put to death. And the status of small bowel of the rats was observed. The immunoglobulin A（ IgA）,CD^3 ＋,CD^4 ＋and CD^8 ＋cell quantity of the small bowel mueosa were detected by immunohistochemical array. Results The villus height,intestinal gland crypt depth and mucosal thickness in the experiment group were better than that of control group（ P〈0. 05）. The quantity of IgA,CD^3 ＋,CD^4 ＋,CD^8 ＋cell of the rats in experiment group were significantly higher than control group（ P〈0. 05）. Conclusion Intestinal immune ecological nutrition can strengthen the rat intestinal immune function and promote restoration of the damage.

关 键 词：免疫营养 生态营养 修复损伤 免疫功能 

分 类 号：R965.1[医药卫生—药理学] R975.1[医药卫生—药学]