特异性敲除T细胞中Blimp-1对小鼠脾淋巴细胞数量及功能的影响  

Effects of T cell-specific Blimp-1 on the number and function of lymphocytes in mice

作　　者：阮素红[1] 赵恺[1] 田宇[1] 袁姝姝[1] 夏洁云 陈翀[1] 徐开林[1] 

机构地区：[1]徐州医学院血液病研究所,徐州医学院附属医院血液科,221002

出　　处：《中华微生物学和免疫学杂志》2015年第7期496-501,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金（81471580,81200376）

摘　　要：目的研究B淋巴细胞诱导成熟蛋白（Blimp-1）对脾脏淋巴细胞数量及功能的影响。方法实验小鼠分为T细胞Blimp-1敲除组（Blimp-1CKO）和对照组（wild-type，WT）。获取小鼠脾脏单个核细胞并计数，流式细胞仪检测T、B淋巴细胞各亚群细胞数、比例及其分泌的细胞因子、表面受体CCR7、S1P1表达。结果与对照组小鼠相比，Blimp-1CKO小鼠脾脏单个核细胞数，T、B淋巴细胞总数及CD4^＋T、CD8^＋T、CD19^＋CD5^＋CD1d^＋B细胞绝对数均明显升高（P〈0．05），Blimp-1CKO小鼠CD19^＋CD5^＋B细胞的数量和比例均显著上升（P〈0．01）。Blimp-1CKO组CD4^＋T和CD8^＋T细胞分泌的IFN-γ、TNF-α、IL-17、IL-2水平较对照组增高（P〈0．05）。Blimp-1CKO小鼠CD8^T细胞表面的CCR7表达较对照组降低（P〈0．05），而两者S1P1表达水平差异无统计学意义。结论Blimp-1对T细胞的数量、亚群平衡、表型、功能的维持都具有重要作用，特异性敲除T细胞Blimp-1对淋巴细胞的影响不仅局限在T细胞，还影响到B细胞亚群。Objective To investigate the effects of B lymphocyte-induced maturation protein-1 （ Blimp-1 ） on the number and function of splenic lymphocytes. Methods The mice with defective Blimp-1 in T cells were generated by cross-breeding B6. Blimp-1flox/flox mice with B6. Lck-Cre mice. The mononuclear lymphocytes isolated from spleen of T cell conditional Blimp-1 knockout （Blimp-1 CKO） mice and wild type （WT） C57/B6 mice were comparatively analyzed. Alterations of CD4^＋T and CD8^＋T cell subsets, the secre- tion of cytokines as well as the expression of C-C chemokine receptor type 7 （ CCR7 ） and Sphingosine-1- phosphate receptor 1 （SIP1） in mice from the two groups were analyzed by flow cytometry. The changes of CD19^＋B cell subsets were also detected. Results Compared with WT mice, the total numbers of mononu- clear cells, T and B lymphocytes were all significantly increased in Blimp-1CKO mice （ P〈0.05 ）. The ab- solute numbers of CD4^＋ T, CD8^＋ T and CD19^＋ CD5^＋ CD1d^＋ B ceils in mice form Blimp-lCKO group were higher than those of the control group （P〈0.05）, however, no significant differences with the percentages of these cell populations were observed between two groups. Higher numbers and percentages of CD19^＋CDS＋B cells were detected in mice from Blimp-lCKO group （P〈0. 01 ）. The Blimp-lCKO mice showed increased secretion of IFN-γ, TNF-α, IL-17 and IL-2, but decreased expression of CCR7 on CD8^＋ T cells as com- pared with WT mice （P〈0.05）. No significant differences with the changes of SIP1 were found between the two groups. Conclusion Blimp-1 played an important role in the maintenance of number, phenotype and function of T cells. Furthermore, not only T cells but also B cell subsets in mice were affected by the dele- tion of Blimp-1 in T ceils.

关 键 词：B淋巴细胞诱导成熟蛋白 T淋巴细胞 B淋巴细胞 

分 类 号：R392[医药卫生—免疫学]