委陵菜积雪草酸对大鼠酒精性肝损伤的保护作用  

Protective effect of asiatic acid from Potentilla chinensis on alcohol hepatic injury in rats

作　　者：赵赶[1] 吕淑娟[2] 韦刚 韦锦斌[2] 林兴[2] 黄权芳[4] 

机构地区：[1]广西壮族自治区妇幼保健院,广西南宁530003 [2]广西医科大学,广西南宁530021 [3]广西分析测试研究中心,广西南宁530022 [4]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中国中药杂志》2015年第14期2866-2870,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81260674,81473431);广西自然科学基金项目(2013GXNSFAA019146,2014GXNSFAA118154)

摘　　要：研究委陵菜积雪草酸（asiatic acid,AA）对大鼠酒精性肝损伤的保护作用及其机制。将Wistar雄性大鼠随机分成正常对照组,AA对照组（8 mg·kg-1AA）,模型组（5.0-9.0 g·kg-1乙醇）,及AA高、中、低剂量治疗组（乙醇＋8,4,2 mg·kg-1AA）。各组灌胃给予相应的药物,每天1次,连续24周。末次给药1.5 h后,取血,处死大鼠,采集肝组织。检测大鼠血清AST,ALT,检测肝脏髓过氧化物酶（MPO）,肿瘤坏死因子（TNF-α）,白细胞介素1（IL-1β）,超氧化物歧化酶（SOD）,GSH-Px,GSH-Rd,丙二醛（MDA）的水平;采用Western blot法检测肝组织中NF-κB,Toll样受体4（TLR4）,CD14,髓样分化因子88（MyD 88）和TIR结构域衔接蛋白-β（TRIF）,蛋白表达情况;同时做组织学检查,观察肝组织的病理变化。结果显示与模型组相比较,AA治疗后,大鼠血清ALT和AST水平,MDA含量明显降低,SOD,GSH-Px,GSH-Rd,MPO活性明显升高。此外,AA能抑制TNF-α,IL-1β的表达;同时还可抑制TLR4,CD14,MyD 88以及核因子NF-κB的水平。组织学检查显示,大鼠肝组织损伤程度均有所减轻。综合以上结果可知委陵菜积雪草酸对乙醇所致的大鼠肝损伤具有一定的保护作用,其机制可能与抑制NF-κB活化减轻炎症反应有关。To study the protective effect and the mechanism of asiatic acid （AA） from Potentilla chinensis on alcohol hepatic injury in rats. Male Wistar rats were randomly divided into six groups： the normal control group, the AA control group （8 mg · kg^-1 AA）, the model group （5.0-9.0 g · kg^-1alcohol） and high, medium and low-dose AA-treated groups （alcohol ＋ 8, 4, 2 mg · kg^-1 AA） Each group was orally administered with the corresponding drugs once a day for 24 weeks. Approximately 1.5 hours after the final administration, all rats were killed, and their blood samples and hepatic tissues were collected. The AST and ALT in rat serum and the contents of MPO, TNF-α, IL-1β, SOD, GSH-Px, GSH-Rd and MDA in hepatic tissues were detected. The expressions of NF-KB, TLR4, CD14, MyD88, TRIF and protein expression in hepatic tissues were measured by western blot. The pathological changes in liver tissues were observed by histological examination. The results showed that compared with the model group, the AA-treated groups showed significant decreases in serum ALT, AST and MDA and increases in the activities of SOD, GSH-Px, GSH-Rd and MPO. Moreover, AA markedly inhibited the expressions of TNF-α, IL-1β, TLR4, CD14, MyD88 and NF-κB. The histological examination showed alleviated hepatic issue ijury to varying degrees. In short, asiatic acid （AA） from P. chinensis could protect alcohol-induced hepatic injury in rats. Its mechanism may be related to the inhibition of NF-κB inactivation and the reduction of inflammatory response.

关 键 词：委陵菜 积雪草酸 酒精 肝损伤 

分 类 号：R285.5[医药卫生—中药学]