玫烟色拟青霉固体培养基筛选及培养条件研究  

Screening of solid culture medium and culture conditions optimization of Paecilomyces fumosoroseus

作　　者：吴丹丹 黄少华 章玉苹 黄华 朱天圣 李传瑛 邓铭光 罗振亚 

机构地区：[1]广东省植物保护新技术重点实验室/广东省农科院植物保护研究所,广东广州510640 [2]广东大丰植保科技有限公司,广东广州510640

出　　处：《广东农业科学》2015年第15期43-47,共5页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2012B010300031);广州市科技计划项目(2014Y2-00513)

摘　　要：玫烟色拟青霉If B01菌株在25℃、RH85%下分别在4种单一培养基(大米、黄豆粉、玉米粉、小麦粉)和3种复合培养基(小麦粉∶麦麸1∶1、黄豆粉∶麦麸1∶1、玉米粉∶麦麸1∶1)培养,以产孢量为指标筛选出较佳的基础培养基配方。通过正交试验验证加入不同浓度的碳源、氮源优化的基础培养基,筛选较佳的固体培养基配方为:玉米粉培养基+0.2%KNO3+0.1%葡萄糖+水(1∶1.5=物料∶水质量比);通过单因素试验,确定菌株较佳的培养条件是28℃、光照黑暗交替培养14h∶10h、产孢前期湿度为80%、产孢后期湿度为50%。Paecilomycesfumosoroseus strain IfB0l was respectively cultured in four single medium （ rice, soybean powder, corn powder and wheatmeal ） and 3 kinds of composite culture medium （ wheat flour ： wheat bran = 1 ： 1, soybean flour ： wheat bran = 1 ： 1 and corn flour ： wheat bran = 1 ： 1 ） at 25℃ and RH85%. Using sporulation as the index, better basal culture medium formula was selected. Through orthogonal experiment, adding different concentrations of carbon source and nitrogen source to optimize basal medium, better solid culture medium formula was as follows ： corn powder basal medium ＋0.2% KNO3＋0.1% glucose ＋ water （ material ： water = 1 ： 1.5 ） . Through single factor experiment, the optimum culture conditions of the strain were as follows： at 28~C and 14h ： 10h （ 1 ： d ） , the humidity was 80% at early stage of spores production, and the humidity was 50% at late stage of spores production.

关 键 词：玫烟色拟青霉 固体培养基 培养条件 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]