鸭源大肠杆菌β-内酰胺类耐药基因的检测  

Detection of β-lactamases-resistant genes in Escherichia coil isolated from ducks

作　　者：嵇辛勤[1,2] 李世静[1,2] 文明[1,2] 张人俊[3] 刘廷江[1] 陈佳琪[1,2] 雷云[1,2] 陈强[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究所,贵州贵阳550025 [3]贵州省动物疫病预防控制中心,贵州贵阳550001

出　　处：《广东农业科学》2015年第15期88-91,共4页Guangdong Agricultural Sciences

基　　金：贵州省科技计划项目(黔科合NY[2013]3074;黔科合计省合[2013]7004)

摘　　要：为了解鸭源大肠杆菌对β-内酰胺类抗生素的耐药性情况,以及β-内酰胺酶类耐药基因的携带情况,从贵州省三穗县部分规模鸭场的患鸭内脏器官中分离的大肠杆菌中随机选取15株,采用药敏纸片法对其进行8种β-内酰胺类药物敏感试验,采用PCR方法对SHV型、CTX-M型、TEM型β-内酰胺酶类耐药基因进行检测,并对耐药基因序列与Gen Bank中的相关序列进行比对。结果显示,15株鸭源大肠杆菌对8种β-内酰胺类抗生素均存在严重耐药,耐药率高达100%。TEM型和CTX-M型耐药基因均有检出,检出率分别为46.7%和100%,而SHV型耐药基因未检出,其中有7株细菌同时带有两种耐药基因。耐药基因序列比对结果显示,所测菌株序列与数据库中相应耐药基因序列相似性均高达90%以上。To understand the resistance of β-1actamases-antibiotics in Escherichia coil isolated from ducks, and the situation of carrying β-1actamases-resistant genes, we randomly selected 15 strains E. coil isolated from ill ducks from some scale duck farms in Sansui county of Guizhou province. The drug sensitive test with 8 kinds of β-1actamases-antibiotics was carried out by drug sensitive slips. The β-lactamases-resistant genes of SHV type, CTX-M type and TEM type were detected by PCR, the sequences of drug resistance genes were analyzed and some relevant sequences in GenBank were compared by DNA Star software. Results showed that serious resistance existed in 15 strains of E. coli to 8 kinds of β-1actamases-antibiotics, the resistance rate was 100%. The TEM type and CTX-M type resistant genes were detected, the detection rate were 46.7% and 100%, while the SHV type resistant gene was none visible. There were 7 strains of bacteria with two kinds of drug-resistant genes at the same time. Sequence alignment results showed that the similarity resistance gene sequences in the database. was above 90% between the tested sequences and corresponding

关 键 词：大肠杆菌 Β-内酰胺类药物 耐药基因 检测 

分 类 号：S852.612[农业科学—基础兽医学]