金果饮的质量控制研究  

Study on Quality Control of Jinguo Yin

作　　者：周雪芳[1] 

机构地区：[1]浙江中医药大学附属湖州市中医院中药房,湖州313000

出　　处：《北方药学》2015年第9期3-5,共3页Journal of North Pharmacy

摘　　要：目的：对金果饮的质量标准进行制定。方法：对玄参、西青果、陈皮建立薄层色谱的鉴别方法;采用高效液相色谱法测定橙皮苷的含量。用Agilent SB C18柱（4.6mm×250mm,5μm）,以甲醇-6%乙酸溶液（35∶65）为流动相;检测波长为283nm,流速1m L·min^-1。结果：薄层色谱鉴别中,供试品在与对照药材色谱相应的位置上,显示相同颜色的斑点,斑点清晰,分离效果好,阴性无干扰;含量测定法中,橙皮苷在36.35-726.95ng（r=0.9999,n=5）范围内呈良好的线性关系,平均加样回收率为98.58%（RSD为1.85%）。结论：建立的方法简便、准确、专属性强、重复性好,能有效用于金果饮的质量控制。Objective：To establish quality control of Jinguo Yin.Methods：TLC method was used to differentiate scrophulariae radix, terminalia chebula retz.and citri pericarpium reticulatae. HPLC method was used to determine content of hespefidin.C18 column （4.6mm× 250mm, 5μm）was used with methanol and 6% acetic acid （35：65） as a mobile phase.The flow rate was 1mL· min^-1,detection wavelength was 283 nm. Results：Samples and controls had the same color fluorescence spots at the same position in the TLC method.Spots were well isolated.ln the HPLC method, linear range was 36.35-726.95ng （r =0.9999, n=5）.Average recovery was 98.58%（RSD=1.85%,n=9）. Conclusion：The established method was sensitive,accurate, repeatable and specific.It can be used for quality control of Jinguo Yin.

关 键 词：金果饮 橙皮苷 质量标准 薄层 高效液相 

分 类 号：R927.2[医药卫生—药学]