云南中华按蚊的遗传变异和种群结构  

Genetic variation and population structure of Anopheles sinensis( Diptera:Culicidae) in Yunnan

作　　者：杨飞龙[1] 李旭东[1] 闫振天[1] 付文博[1] 陈斌[1] 

机构地区：[1]重庆师范大学生命科学学院,昆虫与分子生物学研究所,重庆401331

出　　处：《生态学报》2015年第16期5449-5457,共9页Acta Ecologica Sinica

基　　金："两江学者"计划专项经费;美国国立卫生研究院NIH项目(R01AI095184);国家自然科学基金(31071968;31372265);重庆市科技攻关重点项目(CSTC2012GG-YYJSB80002)

摘　　要：为了掌握云南省各地中华按蚊种群间的遗传变异和种群结构特征,测序并分析了采自云南9个样本点5个种群组的89头中华按蚊的线粒体COII基因。结果表明这些中华按蚊种群的COII基因序列平均单倍型多样性指数和核苷酸多样性指数分别为h=0.933,π=0.00406,共有51个变异位点,占分析的739个碱基总数的6.9%;定义了39个单倍型,有2个频率最高的单倍型H1和H9,分别占个体序列数的20.2%和12.4%;系统发育分析表明单倍型与地理位置没有明显的对应关系,单倍型网络图显示大部分单倍型分布没有明显的亲缘地理格局,主要以单倍型H1、H9、H4、H33和H2为中心呈星状分布,但元江和元阳构成的种群组(YU)单倍型存在明显地域分布特征;AMOVA结果表明种群组间遗传变异为12.58%,达到显著水平(P=0.04888),地理种群组间具有明显种群遗传结构。不同地区两两种群组间的Fst值和Nm值显示大部分种群组间存在基因交流,没有形成明显的遗传分化,但YU种群组和其他种群组间缺乏明显的基因交流,这主要是因为哀牢山的阻隔,使云南东西部形成两种不同的气候,产生了明显的遗传分化;歧点分布图显示为明显单峰分布,中性检测结果均为显著负值,说明云南省的中华按蚊种群在近期经历过复杂的种群扩张事件。掌握中华按蚊遗传多样性及分化特征,对中华按蚊及疟疾控制具有重要的作用。Anopheles sinensis （Diptera： Culicidae） is an important malarial vector in Yunnan Province, China. In order to explore the genetic variation and population structure among different populations of the Species, we sequenced and analyzed mitochondrial COII sequences of 89 An. sinensis samples. These samples were collected from nine localities in Yunnan, and were classified into five population groups based in different geographical locations. The results showed that An. sinensis had high genetic diversity in Yunnan with the haplotype diversity （h） and nucleotide diversity （7r） being 0.933 and 0.00406, respectively. Fifty-one variations were found, occupying 6.9% of the total 739 bp of the COIl sequence. Thirty-nine haplotypes were identified, making up 43.8% of 89 samples, with H1 and H9 being the most widespread, occupying 20.2% and 12.4% of the total haplotypes, respectively. The Jinghong-Mengla population group （JM） has the highest haplotype and genetic diversity, followed by the Yunlong population group （YL）. The tropical rainforest region in Xishuangbanna, which is characterized by high temperature, humidity, and a primary ecological environment, might contribute the most to the high genetic diversity of the JM. Phylogenetic analysis did not reveal a significant relationship between the haplotypes and geographical locations. The haplotype network diagram did not reveal a relative geographical pattern of haplotypedistribution, with the exception of the Yuanjiang-Yuanyang population group （YU） of haplotypes that showed obvious characteristics associated with regional distribution. Haplotypes were mainly distributed around the haplotypes H1, H2, H4, H33, and H2. Analysis of molecular variance （AMOVA） indicated the existence of population structure among population groups. No obvious Fst and Nm values were observed among four population groups （ZYT, YL, S J, and JM） from seven sampling localities, which might indicate that there are no obvious genetic differences and that gene flo

关 键 词：云南省 中华按蚊 MTDNA COⅡ 遗传变异 种群结构 

分 类 号：Q963[生物学—昆虫学]