广州番禺区汉族人群2型糖尿病与Preproghrelin基因rs96217位点单核苷酸多态性的关系  

Relationships between type 2 diabetes mellitus in Han ethnics of Panyu of Guangzhou and single nucleotide polymorphism rs96217 of Preproghrelin gene

作　　者：李舜法 黄建锋[1] 曾莹[1] 焦紫南 崔炳权[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《中国医药科学》2015年第15期9-11,50,共4页China Medicine And Pharmacy

基　　金：广东药学院大学生创新实验项目(1057312036)

摘　　要：目的探讨广州番禺区汉族人群2型糖尿病与Preproghrelin基因rs96217位点单核苷酸多态性的关系。方法提取番禺汉族人群中非糖尿病的正常人和2型糖尿病患者的DNA,采用PCR-RFLP,分析对照组和T2DM组Preproghrelin基因rs96217位点的基因型及组间基因型、等位基因频率分布的差异。结果对照组116例样本中,CC 96例、AC 20例,AA 0例;基因型频率分别为:82.8%、17.2%、0。T2DM组118例样本中,CC 89例、AC 24例、AA 5例,基因型频率分别为:75.4%、20.3%、4.3%。组间基因型和等位基因频率差异无统计学意义。结论广州番禺区汉族人群2型糖尿病与Preproghrelin基因rs96217位点单核苷酸多态性之间未见显著相关性。但T2DM组中发现AA基因型,而对照组未显,原因有待进一步研究。Objective To study the relationships between type 2 diabetes mellitus （T2DM） in Han ethnics of Panyu and SNP rs96217 of preproghrelin gene. Methods Extraction DNA of 116 non-T2DM healthy individuals （the control group） and 118 T2DM individuals （the T2DM group） from populations of Han ethnics in Panyu, target gene segments were detected using PCR-RFLP in order to analyze genotypes of SNP rs96217 of preproghrelin gene, and to compare the differences of genotypic and allelic frequencies between the two groups. Results In the control group, the genotypes CC and AC had 96 and 20 individuals, but genotype AA wasn＇ t find. Their genotypic frequencies were 82.8%,17.2% and 0%, respectivel. In the T2DM group, the genotypes CC, AC and AA were detected in 89, 24 and 5 cases, Their genotypic frequencies were 75.4%,20.3% and 4.3%, respectively, there were no statistical significance in the difference of genotypic and allelic frequencies between the control group and the T2DM group. Conclusion There are no significant relationships between T2DM in Han ethnics of Panyu and SNP rs96217 of preproghrelin gene. However, genotype AA was detected in the T2DM group but not in control, which suggested that allele A may play certain roles in T2DM development. Further investigations certainly would be worth pursuing.

关 键 词：Preproghrelin基因 单核苷酸多态性 2型糖尿病 聚合酶链反应-限制性片段长度多态分析技术 

分 类 号：R587.1[医药卫生—内分泌]