牦牛POMC基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of POMCGene of GBJD Yak

作　　者：陈攀攀[1,2] 柴志欣[1,2] 钟金城[1,2] 

机构地区：[1]动物遗传育种学国家民委-教育部重点实验室,西南民族大学,成都610041 [2]西南民族大学青藏高原研究院,成都610041

出　　处：《西北农业学报》2015年第7期6-15,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西南民族大学研究生创新型科研项目(CX2014SZ128)

摘　　要：为探讨工布江达牦牛POMC基因的分子结构特征,通过分子克隆技术获得工布江达牦牛POMC基因全序列,采用生物信息学方法对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构等进行预测与分析。结果表明,工布江达牦牛POMC基因包含1个798bp的开放阅读框,编码265个氨基酸,其编码蛋白属于亲水性蛋白,具有明显的信号肽,二级结构主要以无规卷曲和α-螺旋为主。以POMC基因构建的邻接系统发育树表明,工布江达牦牛POMC与野牦牛、普通黄牛、水牛和羊等品种的POMC遗传距离较近,具有高度同源性。In order to explore the structures of POMC gene of GBJD yak, the complete sequence of GBJD yak POMC gene was cloned by molecular cloning technique, Some characters of POMC gene and its encoded protein sequences were predicted and analyzed by methods in bioinformatics, such as the general physical and chemical properties, hydrophobicity, signal peptide and secondary structure. The results showed that the full-length of GBJD yak POMC gene contained a complete ORF（798 bp）, which could encode 265 amino acid, the protein encoded by yak POMC gene had obvious signal peptide, it was a hydrophilic protein, the secondary structure was mainly composed of Helix and Coli. The POMC gene of GBJD yak, Bos mutus, Bos taurus, Bubalus bubalis and Ovis were close in phylogenetic tree,which had high homology.

关 键 词：牦牛 POMC基因 克隆 生物信息学分析 

分 类 号：S823[农业科学—畜牧学] Q78[农业科学—畜牧兽医]