甘蓝花蕾小孢子发育同步性影响因素初步探讨  

Preliminary Exploration of Factors Affecting Development Synchronicity of Microspores in Brassica oleracea L.Buds

作　　者：王鑫[1] 刘争[1] 张恩慧[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2015年第7期113-118,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家科技支撑计划项目(2012BAD02B01);陕西省科学技术研究统筹项目(2012KTCL02-09)

摘　　要：为了提高甘蓝单花蕾小孢子发育同步性,完善游离小孢子培养技术体系,对小孢子供体植株通过整枝、NAA处理和增施微肥3种处理对其影响因素进行初步探究。结果表明,只留一级侧枝(CL2)能有效提高花蕾小孢子发育同步性;1 000mg/L(N2)质量浓度的NAA处理后也能显著提高花蕾小孢子发育同步性;增施质量分数0.1%的微肥后,单核靠边期最大比率可达76.0%,比对照(35.6%)有极显著的提高。In order to improve microspore development synchronicity in single bud of Brassica oleracea L.and optimize the technological system of culturing the isolated microspores,the study evaluated the effect of artificial pruning,spraying NAA and fertilizing micronutrients on the microspore development of the donor buds.The results showed that only keeping the primary lateral branch（CL2）or treated by 1 000 mg/L（N2）can improve microspore development synchronicity in buds effectively.After applying 0.1% micronutrient fertilizer,the biggest ratio of microspores at telophase I was up to76.0%,which is 35.6% higher than the control.

关 键 词：甘蓝 小孢子 单核靠边期 发育同步性 

分 类 号：S635[农业科学—蔬菜学]