四川小麦品种抗条锈基因的SSR分析  

The SSR Analysis of Resistance Gene to Stripe Rust in Sichuan Wheat Varieties

作　　者：康晓慧[1] 彭玉姣[1] 付菊梅[1] 白雪[1] 张春容[1] 蔚慧欣 

机构地区：[1]西南科技大学生命科学与工程学院,中国绵阳621010

出　　处：《湖南师范大学自然科学学报》2015年第3期11-15,共5页Journal of Natural Science of Hunan Normal University

基　　金：公益性行业(农业)科研专项资助项目(200903035)

摘　　要：从46对小麦基因组SSR引物中,筛选出带型清晰、稳定、多态性丰富的引物11对.利用该11对引物对52份抗病材料进行了遗传多样分析.结果表明,11对SSR引物共扩增出66个多态性位点,其中,每对SSR有1~10个,平均为6个.位点多态性指数（PIC）为0.75~0.90,平均为0.84.UPGMA聚类分析表明,SSR标记可将52份抗病品种分为3大类7个亚类.本研究对抗条锈基因功能分析及小麦抗条锈病分子辅助育种有参考价值.11 pairs of SSR primer with clear, steady and obviously polymorphic bands were screened from 46 pairs of SSR primer in wheat genome. The genetic diversities of 52 wheat cuhivars with stripe rust resistance were analyzed by using 1 ! SSR primer pairs. The results showed that 11 pairs of primer generated 66 polymorphic sites and each primer amplified 1 to 10 sites with a mean of 6. The polymorphism information content （PIC） of 11 pairs ranged from O. 75 to 0.90 with an average of 0.84. UPGMA cluster analysis indicated that the germplasms could be divided into 3 large groups and 7 cluster subgroups based on SSR markers. The research has some reference value for the function analysis of resistance genes to stripe rust and molecular marker assisted breeding.

关 键 词：小麦 抗病基因 条锈菌 SSR 

分 类 号：S432.1[农业科学—植物病理学]