盐胁迫下盐穗木甘油醛-3-磷酸脱氢酶基因的表达与亚细胞定位分析  

Subcellular Localization and Expression of Glyceraldehyde-3-phosphate Dehydrogenase Gene from Halostachys caspica under Salt Stress

作　　者：张霞[1,2] 张桦[1] 张富春[2] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆大学生命科学与技术学院,新疆生物资源与基因工程重点实验室,乌鲁木齐830046

出　　处：《西北植物学报》2015年第7期1283-1288,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：973计划前期研究专项(2012CB722204)

摘　　要：为研究盐胁迫下植物甘油醛-3-磷酸脱氢酶(GAPDH)的作用机制,该研究利用RACE技术,从藜科盐生植物盐穗木中克隆获得1 381bp GAPDH基因(HcGAPDH)全长cDNA序列,其开放阅读框(ORF)编码314氨基酸。序列分析表明,HcGAPDH属于胞质GAPDH家族成员。实时定量PCR结果显示,HcGAPDH的转录水平受盐胁迫和ABA上调。通过荧光共聚焦显微技术,检测到绿色荧光蛋白标记的HcGAPDH在正常情况下定位于细胞质中,盐胁迫可促使其从细胞质转移至细胞核中,此过程与细胞内碳水化合物代谢的信号途径不具有相关性。研究表明,HcGAPDH通过细胞质和细胞核之间的信号传导在植物盐胁迫中发挥一定作用,为进一步揭示盐穗木耐盐分子机制奠定了一定基础。To study the precise roles of cytosol GAPDH in plant response to salt stress,we obtained 1 381 bp HcGAPDH from Chenopodiaceae uhalophyte Halostachys caspica with RACE technology.The ORF（open reading frame） of which encode 314 amino acids.HcGAPDH gene displayed considerable homology with known members of the GAPDH protein family.The expression of HcGAPDH is upregulated by salt stress and abscisic acid treatment.Green fluorescent protein- tagged HcGAPDH was localized in cytosol of the transgenic Arabidopsis thalinana cells,and could transfer into nucleus under salt stress,which is independent of carbohydrate- mediated signaling.Our results implicate that HcGAPDH function in plant cells through modulating cytosol to nucleus-signaling cascade under salt stress.The study laid a certain foundation for elucidation of salt-tolerance mechanism of Halostachys caspica at molecular level.

关 键 词：盐穗木 甘油醛-3-磷酸脱氢酶 盐胁迫 亚细胞定位 细胞质 细胞核 

分 类 号：Q785[生物学—分子生物学] Q786