古代三合土灰浆中蛋清的酶联免疫检测研究  

Research on the Egg White in Ancient Tabia Mortar by Enzyme-Linked Immunosorbent Assay(ELISA)

作　　者：张秉坚[1,2] 胡文静[1] 张坤[2] 方世强[1] 

机构地区：[1]浙江大学化学系,浙江杭州310027 [2]浙江大学文物与博物馆学系,浙江杭州310027

出　　处：《建筑材料学报》2015年第4期716-720,共5页Journal of Building Materials

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB720902);"十二五"国家科技支撑计划项目(2012BAK14B05)

摘　　要：蛋清灰浆三合土(简称蛋清灰浆)是中国古代广泛应用的一种重要建筑灰浆,了解其成分不仅是研究中国建筑科技史的需要,对于濒危文物建筑的维修加固等也具有十分重要的意义.利用抗原-抗体免疫反应的高特异性和灵敏度,采用酶联免疫吸附法(ELISA),首次实现了蛋清灰浆中微量蛋清成分的准确检测,最低检出浓度(质量分数)可达0.003%,同时解决了灰土本身的颜色干扰问题,为研究古代蛋清类灰浆的成分提供了有效检测技术.Egg white tabia mortar（referred to as ＂egg white mortar＂） as the representative of the ancient traditional mortar, was widely used in different areas in China. Determination of the composition of cemen ting material left in cultural relics, is of importance not only for Chinese architecture history and art tech- nology history, but also for maintenance of endangered cultural relics and development of modern green ce- menting material. High specificity and sensitivity of antigen-antibody immunoreaction were utilized. Accu- rate detection of micro-egg white in ancient mortar samples by enzyme-linked immunosorbent assay （ELISA）, have been realized for the first time. The minimum detectable concentration was 0. 003%（by mass）. The interference from the color of earth itself is avoided, providing the effective detecting tech- niques for the determination of the composition of ancient tabia mortars and other remains.

关 键 词：蛋清灰浆 检测分析 酶联免疫吸附法 

分 类 号：TU578[建筑科学—建筑技术科学]