玄参饮片质量控制方法研究  

Study on Quality Control Method of Radix scrophulariae Pieces

作　　者：贾成友[1,2] 张传辉[2] 赵凤平[1] 傅亚[3] 王云红[2] 杨荣平[1,2] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]重庆市中药研究院,重庆400065 [3]重庆科技学院化学化工学院,重庆401331

出　　处：《天然产物研究与开发》2015年第8期1385-1390,共6页Natural Product Research and Development

基　　金：国家科技支撑计划(2011BAI13B03)

摘　　要：研究中药质量评价中一测多评与指纹图谱结合的考察模式，以重庆市售玄参饮片为研究对象，以肉桂酸为参照物建立玄参饮片HPLC指纹图谱及其与其余成分的相对校正因子，并计算其含量，实现一测多评。结合建立的玄参HPLC指纹图谱，对饮片进行相似度评价，并进行聚类分析和主成分分析。结果建立了玄参饮片HPLC指纹图谱，标定了18个共有峰，指认了其中3个共有峰，11批饮片相似度在0.731～0.960之间，16批饮片中只有11批饮片含量符合药典规定，并将其有效地区分为三类。结果表明一测多评结合指纹图谱的质量控制模式适合于玄参饮片质量评价，重庆市售玄参饮片质量参差不齐，应加强中药饮片的规范化生产。To establish a new method for quality evaluation of traditional Chinese medicine by fingerprinting analysis and quantitative analysis of multi-components by single marker method （QAMS）.The quality evaluation method was established and validated with the prepared pieces of Radix scrophulariae that were commercially available in Chongqing.Cinnamic acid was selected as markers of ingredients to establish HPLC fingerprint and was used as internal reference standard to determine the contents of other 2 components （harpagide,harpagoside） according to the relative correction factor.HPLC fingerprint of R.scrophulariae was finally established.All the tested samples contained 18 common characteristic peaks,3 of which was verified to be harpagide,cinnamic acid and harpagoside.Only 11 of the 16 batches samples conformed to the requirement of pharmacopoeia of Chinese.The similarities of 11 batches of prepared pieces of R.scrophulariae were between 0.731-0.960.In addition,the principal component analysis （PCA） showed that samples were divided into 3 groups efficiently.The method of QAMS combined with fingerprinting analysis was to be a new quality evaluation pattern for R.scrophulariae pieces.The quality of prepared pieces of R.scrophulariae commercially available in Chongqing was uneven.Accordingly,production procedures should be normalized.

关 键 词：指纹图谱 含量测定 聚类分析 主成分分析 玄参 

分 类 号：R284[医药卫生—中药学]