银杏叶提取物对胃癌SGC7901细胞增殖与凋亡的影响  

Effects of Ginkgo biloba Extract on Proliferation and Apoptosis of Gastric Cancer Cell Line SGC7901

作　　者：陈海挺 胡江飚 卢敏攀 

机构地区：[1]永康市第一人民医院中医康复科,浙江永康321300

出　　处：《中国药房》2015年第25期3510-3512,共3页China Pharmacy

摘　　要：目的:研究银杏叶提取物(GBE)对胃癌SGC7901细胞增殖与凋亡的影响。方法:0、5、10、20、50、100、200μg/ml GBE培养细胞48 h后,MTT法测定细胞活力并计算抑制率。0、10、50、200μg/ml GBE培养细胞48 h后,流式细胞仪检测细胞周期分布及凋亡率。结果:5、10、20、50、100、200μg/ml GBE培养细胞48 h后,对细胞生长有明显抑制作用,且呈剂量依赖关系。10、50、200μg/ml GBE培养细胞48 h后细胞凋亡率升高。结论:GBE可一定程度上抑制SGC7901细胞增殖,诱导其凋亡,以前者为主。OBJECTIVE：To investigate the effects of Ginkgo biloba extract（GBE） on proliferation and apoptosis of gastric cancer cell line SGC7901. METHODS：After the cells were cultured in 0,5,10,20,50,100 and 200 μg/ml GBE for 48 h,cell viability was determined by MTT method and inhibition rate was calculated. After the cells were cultured in 0,10,50 and 200μg/ml GBE for 48 h,flow cytometry was used to detect cell cycle distribution and the apoptosis rate. RESULTS：Following 48 h culture of cells in 5,10,20,50,100 and 200 μg/ml GBE,the growth of cells were obviously inhibited in a dose- dependent manner. After 48 h culture of cells in 10,50 and 200 μg/ml GBE,the apoptosis rate was increased. CONCLUSIONS：GBE can inhibition the proliferation of SGC7901,and induces it apoptosis,the former dominated.

关 键 词：银杏叶提取物 胃癌SGC7901细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]