侧柏叶水煎液对耳廓炎症和腹腔炎症模型小鼠的抗炎作用  

Anti-inflammation Effects of Cacumen Biotae Water Decoction on Mice with Auricle and Abdominal Inflammation

作　　者：李丽[1] 苗文静 王青[3] 

机构地区：[1]济宁医学院基础医学教研室,山东日照276826 [2]日照市妇幼保健院检验科,山东日照276826 [3]日照市人民医院,山东日照276826

出　　处：《中国药房》2015年第25期3515-3517,共3页China Pharmacy

摘　　要：目的:研究侧柏叶水煎液对耳廓炎症和腹腔炎症模型小鼠的抗炎作用。方法:将小鼠随机分为正常对照组、模型组、阳性对照(阿司匹林0.25 g/L)组和侧柏叶高、中、低剂量[1、0.5、0.25 g(生药)/L]组,依次记为A、B、C、D、E、F组,每组10只,连续ig给药5 d。除A组外,其余各组分别复制耳廓炎症模型和腹腔炎症模型。检测耳廓炎症模型小鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)含量,计算肿胀度和肿胀率;检测腹腔炎症模型小鼠肝组织中TNF-α、IL-1、酸敏感离子通道1a(ASIC1a)含量和腹腔灌洗液中蛋白质浓度(ΔA)。结果:耳廓炎症模型中,与A组比较,B组小鼠血清中TNF-α、IL-1含量增加;与B组比较,C、D、E、F组小鼠血清中TNF-α、IL-1含量减少,肿胀度和肿胀率降低;与C组比较,E、F组小鼠血清中TNF-α、IL-1含量、肿胀度和肿胀率增加;以上差异有统计学意义(P<0.05)。腹腔炎症模型中,与A组比较,B组小鼠肝组织中TNF-α、IL-1、ASIC1a含量和ΔA均增加;与B组比较,C、D、E、F组小鼠TNF-α、IL-1、ASIC1a含量和ΔA降低;以上差异有统计学意义(P<0.05)。结论:侧柏叶水煎液对耳廓炎症和腹腔炎症模型小鼠有抗急性炎症作用,其机制可能与降低TNF-α、IL-1及ASIC1a有关。OBJECTIVE：To study the anti-inflammation effects of cacumen biotae water decoction on mice with auricle and abdominal inflammation. METHODS：Mice were randomized into a normal control group,a model group,a positive control group（0.25 g/L aspirin）and cacumen biotae high-dose,medium-dose and low-dose [1,0.5 and 0.25 g（medicinal materials）/L] groups,respectively marked as groups A,B,C,D,E and F,with 10 mice in each group. The mice were given drugs ig for 5 consecutive days. The models of auricle and abdominal inflammation were established for all groups except the normal control group. The contents of tumor necrosis factor α（TNF-α）and interleukin 1（IL-1）in the serum of mice with auricle inflammation were determined,and the swelling degree and swelling rate were calculated. The contents of TNF-α,IL-1 and acid-sensing ion channels 1a（ASIC1a）in the liver tissues of mice with abdominal inflammation and the concentration of proteins in the peritoneal lavage fluid（ΔA）thereof were detected. RESULTS：For mouse models of auricle inflammation,the contents of TNF-α and IL-1 in serum in group B were higher than in group A;the swelling degree and swelling rate and the contents of TNF-α and IL-1 in groups C,D,E and F were lower than in group B,and those of group E and F were higher than in group C. There were statistical differences（P〈0.05）. For mouse models of abdominal inflammation,the contents of TNF-α,IL-1 and ASIC1 ain liver tissues and ΔA in group B were higher than in group A;the contents of TNF-α,IL-1 and ASIC1 aas well as Δ A in groups C,D,E and F were lower than in group B.There were statistical differences（P〈0.05）. CONCLUSIONS：Cacumen biotae water decoction has anti-effect on acute auricle and abdominal inflammation by a mechanism which may be related to decreasing TNF-α,IL-1 and ASIC1 a.

关 键 词：侧柏叶 耳廓炎症 腹腔炎症 急性炎症 酸敏感离子通道1a 小鼠 

分 类 号：R285[医药卫生—中药学]