多个月季品种与蔷薇的花粉生活力研究  

The Research on the Pollen Viability of Rosa chinensis Varieties and Rosa multiflora with Different Methods

作　　者：杨攀 王升 郭风民 李永华[2] 

机构地区：[1]郑州市城市园林科学研究所,郑州450051 [2]河南农业大学林学院,郑州450002

出　　处：《河南科学》2015年第8期1329-1332,共4页Henan Science

基　　金：郑州市技术研究重点攻关项目(141PZDGG210)

摘　　要：以30个月季品种及蔷薇属植物为试验材料,采用沙尔达考夫染色法、悬滴液发芽法测定其花粉生活力,并根据花粉萌发率对品种进行分级.结果表明：1）悬滴液发芽法更适合花粉生活力的测定,10%蔗糖浓度下适宜月季品种及蔷薇属植物的花粉萌发;2）30个月季品种和蔷薇中,花粉萌发率较高的是‘十姊妹’、‘橘红火焰’、‘小果蔷薇’和‘粉荷’,分别为84.20%、71.60%、59.20%和54.00%,花粉生活力随着时间的延长而降低;3）以花粉萌发率为判断标准,月季品种及蔷薇分为三个等级,一级品种：‘十姊妹’、‘橘红火焰’,萌发率高于50%;二级品种：‘粉和平’、‘摩尼卡’,萌发率在10%~50%;三级品种：‘光谱’、‘金阵雨’,萌发率低于10%.With a total of 30 Rosa chinensis varieties and Rosa multiflora as test materials,the pollen viability was measured by staining and germination,and the varieties was graded according to the pollen germination. The result showed as follows. The germination was suitable for detecting pollen viability,the measurement with 10%sucrose concentration was suitable for the Rosa chinensis and Rosa multiflora varieties pollen germination.‘Rosa multiflora Thunb.var.platyphylla Thory’‘Darthuizer Orang Fire’‘Rosa cymos’and‘Fen He’had the higher pollen germination rate than other varieties,and the rates respectively were 84.20%,71.60%,59.20%and 54.00%,the pollen viability gradually reduced as the extension of storage time. With the pollen germination as the judgment ,Rosa chinensis and Rosa multiflora varieties were classified three grades：the pollen germination of the first grade was more than 50%,‘Rosa multiflora Thunb.var.platyphylla Thory’‘Darthuizer Orang Fire’;the second grade was between 10% and 50%‘,Pink Peace‘’Monica’;the third grade was less than 10%‘,Spectra‘’ Golden Showers’.

关 键 词：月季 蔷薇 花粉生活力 沙尔达考夫染色 悬滴液发芽法 

分 类 号：S685.12[农业科学—观赏园艺]