光质对离体培养条件下绿色棉纤维发育及黄酮合成的影响  

Effects of Light Quality on Fiber Development and Flavonoid Synthesis of Green Cotton in vitro Culturing

作　　者：洪亮[1] 张雷[1] 钱森和[1,2] 孙旭[1] 蔡永萍[1] 林毅[1] 高俊山[1] 郭宁[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽工程大学生化学院,芜湖241000

出　　处：《棉花学报》2015年第4期300-309,共10页Cotton Science

基　　金：安徽省11.5棉花创新团队;安徽省棉花产业技术体系;安徽省教育厅重点项目(KJ2011A103;KJ2012A117);安徽省自然科学基金(KJ2014A078)

摘　　要：为探讨光质对绿色棉纤维生长发育和黄酮合成的影响,以绿色棉品种绿絮棉1号为材料进行离体培养,比较红光、黄光、蓝紫光、白光和暗处理条件下绿色棉胚珠鲜物质质量、纤维长度、纤维素含量、黄酮含量等生理和分子指标的差异。结果表明：光处理能够促进胚珠鲜物质质量的增加,但抑制了纤维的伸长。在纤维发育前期,各光处理抑制了蔗糖合成酶活性和蔗糖合成酶基因表达;纤维发育后期,白光处理提高了蔗糖合成酶活性,促进了蔗糖合成酶基因表达。红光、黄光和蓝紫光处理显著提高了β-1,3-葡聚糖酶活性（10~20 DAC（Days after culture））;红光、黄光和白光均能提高β-1,3-葡聚糖基因表达量,而蓝光则抑制了基因的表达。白光促进了纤维素的合成,而蓝紫光抑制了纤维素的合成。在纤维发育初期（10 DAC）,蓝紫光抑制了黄酮合成及其关键酶基因表达;而在发育后期（30~40 DAC）,白光处理能够促进黄酮的合成及其关键酶基因表达。可见,光质对绿色棉纤维发育及黄酮合成具有一定的影响。To investigate the effect of light quality on green cotton fiber development and flavonoid synthesis in the green cotton variety Lüxumian I, physiological and molecular indicators were measured as a materials, and the ovule fresh weight, fiber length, cellulose content, flavonoids content and other physiological and molecular indicators with red light, yellow light, blue light, white light, and dark growth conditions. All light conditions tested promote ovule fresh weight, while inhibiting fiber elongation. During early fiber development, all light conditions inhibit sucrose synthase activity and sucrose synthase Su Sy gene expression. Only white light improves sucrose synthase activity and promotes Su Sy expression at a later stage in fiber development. Red, yellow, and blue light significantly increased β-1,3-glucanase activity（10-20 Day after culture, DAC）, while red, yellow, and white light improved β-1,3-glucanase gene expression. Only blue light suppressed expression at this locus.Cellulose synthesis is promoted by white light and inhibited by blue light. During early fiber development（10 DAC）, blue light inhibits flavonoid synthesis and expression of key genes in this pathway. During late fiber development（30-40 DAC）, white light promotes flavonoid synthesis and key gene expression. Thus, these finding suggest light quality has a certain influences on the fiber development and flavonoid synthesis of green cotton.

关 键 词：绿色棉 离体培养 光质 基因表达 纤维发育 黄酮合成 

分 类 号：S562[农业科学—作物学]