TD70/Kasalath RIL群体定位水稻花药基部开裂长度QTL  

Mapping of QTLs for length of basal dehiscence of thecae in rice using TD70/Kasalath RIL population

作　　者：赵凌[1] 张亚东[1] 赵春芳[1] 周丽慧[1] 姚姝[1] 于新[1] 丁丹[1] Tsutomu Matsui 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,江苏南京210014 [2]Faculty of Applied Biological Science,Gifu University

出　　处：《江苏农业学报》2015年第4期711-717,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31201181);江苏省农业科技自主创新基金项目[CX(13)5004]

摘　　要：利用花药基部开裂长度存在明显差异的粳稻品系TD70和籼稻品种Kasalath的重组自交系(包含240个系)为作图群体,对控制水稻花药基部开裂长度的QTL进行鉴定,并对水稻花药基部开裂长度和其他农艺性状进行相关性分析。利用Ici Mappingv3.4软件,运用复合区间作图法,以均匀分布于12条染色体的141个SSR标记进行花药基部开裂长度的QTL检测,共检测到2个QTL位点,其中q LDBT6位于第6染色体RM3和RM3628之间,LOD值3.23,贡献率6.18%,加性效应17.74%;q LDBT8位于第8染色体RM1376和RM4085之间,LOD值2.95,贡献率8.00%,加性效应20.09%,推测该区域与小穗不育有重要关系,有必要进一步研究。RIL群体的花药基部开裂长度与剑叶宽、千粒质量、粒长、粒宽、粒厚间存在极显著正相关,与抽穗期、株高、分蘖数、剑叶长、总粒数、结实率、抽穗当天最高温度、抽穗期平均温度、抽穗期间最高温度以及芒长之间不相关。Two hundred and forty recombinant inbred lines ( RIL ) derived from a cross between japonica rice TD70/Kasalath and its linkage map including 141 SSR markers were used to map QTLs controlling length of basal dehiscence of thecae(LDBT). Two QTLs controlling LDBT were detected on chromosomes 6 and 8, respectively. qLDBT6 was mapped between the marker RM3 and RM3628 on chromosome 6 with the LOD value of 3. 23. qLDBT8 was mapped between the mark-er RM1376-RM4085 on chromosome 8 with the LOD value of 2. 95. The LDBT of RILs had significant positive correlations with width of flag leaf, grain weight, grain width,grain length and grain thickness. No significant cor-relation was found between LDBT and plant height,heading date,number of tillers,length of flag leaf,average tempera-ture at heading, and maximum temperature at heading, etc.

关 键 词：水稻 重组自交系 花药基部开裂长度 QTL 

分 类 号：S511[农业科学—作物学]