苏丹草管状分子体外诱导及木质素合成相关酶活力的变化  

In vitro induction of tracheary elements of Sorghum sudanense and changes in lignin synthesis related enzymes activities

作　　者：刘兆明[1,2] 钟小仙[2] 钱晨[2] 刘大林[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省农业科学院畜牧研究所,江苏南京210014

出　　处：《江苏农业学报》2015年第4期846-854,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家牧草产业技术体系综合试验站项目(CARS-35);江苏省农业科技自主创新基金项目[CX(13)3035];江苏省第四期"333工程"培养资金资助项目

摘　　要：为探索C4作物次生细胞壁合成机制,以苏丹草新品系苏牧3号的胚性愈伤组织为材料,研究了光照、外源植物激素和活性炭对愈伤组织管状分子分化的影响,并对管状分子诱导过程中木质素含量、可溶性酚醛组分含量及其相关酶活力变化进行了分析。结果表明,在每天光照16 h、不含外源植物激素的基本培养基中添加3 g/L活性炭,诱导培养20 d,管状分子分化率最高,为51.97%,管状分子出现的起始时间为诱导培养第4 d;诱导培养21 d时,木质素含量达到最高值18.11%,与木质素合成相关的酶PAL、CAD活力也同时达到最高值,分别为5.915 0×10-3U/mg、8.366 7×10-2U/mg,此后木质素含量和PAL、CAD酶活力均无显著变化;POD活力诱导培养9 d时达到最高值132.24 U/mg,显著高于继代培养;可溶性酚醛组分含量先增后减,诱导培养12 d时达到最高值1.09μg/mg。To reveal the secondary cell wall synthesis mechanism of C4 crops, the effect of illumination, exoge-nous hormone and activated carbon concentration on tracheary elements induction in vitro was studied on sudangrass Sumu No. 3 . The changes of lignin content, soluble phenolic components contents and related enzymes activities were analyzed in the process of tracheary elements induction as well. The results showed that the differentiation rate of tracheary elements reached the highest point (51. 97%) at 20th day induction in 16-h daily illumi-nation when calli were cultured in plant hormone-free MS medium with 3 g/L activated carbon addition. After 21-d induction, ligin content get to the peak value of 18. 11%, and so did the activities of enzymes PAL <br> (phenylalanine ammonia lyase and CAD (cinnamyl alcohol dehydrogenase, which were shown to be 5.915 0×10-3 U/mg and 8. 366 7×10-2 U/mg, respectively. The lignin content and enzymatic activities did not changed significant-ly afterwards. The activity of enzyme POD (peroxidase) came to its maximum value of 132. 24 U/mg after 12-d in-duction. Soluble phenolic components content increased over time and reached the highest of 1. 09 μg/mg after 12-d induction 12th day, and then decreased.

关 键 词：苏丹草 管状分子 体外诱导 木质素合成 

分 类 号：S544.1[农业科学—作物学]