海洋真菌提取物损伤大肠杆菌DNA活性的筛选及活性菌株研究  

Screening the Extracts of Marine Fungi for the Activity of Damaging the DNA of Escherichia coli and Researches on Active Strains

作　　者：鲍海燕[1] 谷朋娟[1] 张翼[2] 穆军[3] 冯妍[2] 赵辰燕[1] 

机构地区：[1]大连交通大学环境与化学工程学院,大连116028 [2]广东海洋大学食品与科技学院广东省水产品加工与安全重点实验室水产品深加工广东普通高等学校重点实验室,湛江524088 [3]浙江海洋学院海洋科学与技术学院,舟山316022

出　　处：《生物技术通报》2015年第8期132-139,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(20902009);中国博士后科学基金项目(2011M500051;2012T50258);广东省"扬帆计划"引进紧缺拔尖人才项目(201433009);广东海洋大学引进人才科研启动项目(E15155);广东海洋大学创新强校工程项目(GDOU2014030502;GDOU2014050203);广东海洋大学自然科学研究项目(C14519)

摘　　要：从海洋微生物中筛选抗菌、抗肿瘤物质是海洋药物研究的热点之一,而筛选模型在研究中起着重要作用。建立了可用于抗菌及潜在抗肿瘤活性物质筛选的大肠杆菌差异性DNA修复试验模型,并运用该模型对海洋共附生真菌的提取物进行了筛选。结果显示,5株海洋真菌的提取物具有较强的选择性抑制DNA损伤修复基因缺陷型大肠杆菌的活性,可能具有DNA损伤作用。对这5种活性真菌进行了分子生物学鉴定,而且薄层色谱(TLC)和高效液相色谱(HPLC)-活性追踪显示这些菌株可产生较多样化的活性物质。Screening of antimicrobial and antitumor agents from marine microorganisms is one of the hot spots for the research of marine drugs, and screening models play an important role in the study. The authors set up an Escherichia coli differentiated DNA damage repair test model, which can be used for the screening of antimicrobial and potential antitumor substances. This model was further applied to screen the bioactivities of the extracts of marine symbiotic fungi. As the result, five marine fungal strains＇ extracts showed strong selective inhibition to E. coli strains with DNA damage repairing genes-deficiency, i.e, the potential of damaging DNA. The five strains were identified by molecular biological methods. Furthermore, analyses by thin layer chromatography （ TLC ）and high performance liquid chromatography（ HPLC ）-bioactivity tracing revealed that these strains produced diverse bioactive substances.

关 键 词：海洋微生物 大肠杆菌差异性DNA修复试验模型 鉴定 

分 类 号：R285[医药卫生—中药学]