空泡形成毒素影响禽致病性大肠杆菌的生物学特性及致病性  

Vacuolating autotransporter toxin affects biological characteristics and pathogenicity of avian pathogenic Escherichia coli

作　　者：赵益超[1,2] 王少辉[2] 杨登辉[2] 刘新[1,2] 韩先干[2] 田明星 丁铲[2] 刘宗平[1] 于圣青[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《微生物学报》2015年第9期1208-1214,共7页Acta Microbiologica Sinica

基　　金：公益性农业(科研)专项(201303044);国家自然科学基金(81201266,31370045);中央级科研院所基本科研业务费项目(2015JB08);上海市科技兴农重点攻关项目[沪农科攻字(2015)第1-9号]~~

摘　　要：【目的】构建禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)菌株APEC-O1空泡形成毒素(Vacuolating autotransporter toxin,Vat)基因缺失株,研究该基因对APEC-O1生物学特性及致病性的影响。【方法】采用Red同源重组的方法构建vat缺失株,并利用低拷贝质粒p STV28构建互补株,然后比较野生株、vat缺失株与互补株在生长特性、运动性、凝集沉淀能力、生物被膜、致病性等生物学特性的差异。【结果】vat基因缺失不影响APEC-O1的生长速度及抗环境压力能力,但缺失vat导致APEC-O1运动能力增强,而使生物被膜形成能力、凝集沉降能力、致病力、体内存活能力均显著性减弱。【结论】Vat缺失影响APEC-O1运动能力、凝集沉淀能力、生物被膜形成能力及致病力,为全面了解Vat的致病作用提供参考。[ Objective ] To determined the role of vacuolating autotransporter toxin （vat） gene in avian pathogenic Escherichia coli （APEC） , we detected the biological characteristics and pathogenicity of vat gene mutant strain of APEC- O1. [ Methods] We constructed the vat mutant and complementary strain of APEC-O1 by the Red recombination system and plasmid pSTV28. Then we compared the growth curve, motility, agglutination, biofilm formation and pathogenicity of mutant strain, wild-type strain and complementary strain. [ Results] The vat mutant did not affect the growth and resistance to environment stress of APEC. However, inactivation of APEC-O1 vat gene resulted in enhanced motility, diminished agglutination, decreased biofilm formation and attenuated virulence in ducks. [ Conclusion ] These data indicated that Vat affect the motility, agglutination, biofilm formation and virulence of APEC-O1, which help us to understand the role of the Vat in the APEC pathogenicity.

关 键 词：禽致病性大肠杆菌 空泡转运毒素 基因缺失 生物学特性 致病性 

分 类 号：R378[医药卫生—病原生物学]